System analysis of Lipomyces starkeyi during growth on various plant-based sugars.
Anshu Deewan and Jing-Jing Liu contributed equally to this work
Lipomyces starkeyi
Metabolomics
Oleaginous yeast
RNA sequencing
Sugar uptake
Transcriptomics
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
04
03
2022
accepted:
16
07
2022
revised:
14
07
2022
pubmed:
30
7
2022
medline:
31
8
2022
entrez:
29
7
2022
Statut:
ppublish
Résumé
Oleaginous yeasts have received significant attention due to their substantial lipid storage capability. The accumulated lipids can be utilized directly or processed into various bioproducts and biofuels. Lipomyces starkeyi is an oleaginous yeast capable of using multiple plant-based sugars, such as glucose, xylose, and cellobiose. It is, however, a relatively unexplored yeast due to limited knowledge about its physiology. In this study, we have evaluated the growth of L. starkeyi on different sugars and performed transcriptomic and metabolomic analyses to understand the underlying mechanisms of sugar metabolism. Principal component analysis showed clear differences resulting from growth on different sugars. We have further reported various metabolic pathways activated during growth on these sugars. We also observed non-specific regulation in L. starkeyi and have updated the gene annotations for the NRRL Y-11557 strain. This analysis provides a foundation for understanding the metabolism of these plant-based sugars and potentially valuable information to guide the metabolic engineering of L. starkeyi to produce bioproducts and biofuels. KEY POINTS: • L. starkeyi metabolism reprograms for consumption of different plant-based sugars. • Non-specific regulation was observed during growth on cellobiose. • L. starkeyi secretes β-glucosidases for extracellular hydrolysis of cellobiose.
Identifiants
pubmed: 35906440
doi: 10.1007/s00253-022-12084-w
pii: 10.1007/s00253-022-12084-w
doi:
Substances chimiques
Biofuels
0
Lipids
0
Sugars
0
Cellobiose
16462-44-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5629-5642Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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