CRISPR/Cas12a-based fluorescence immunoassay: combination of efficient signal generation with specific molecule recognition.

Biosensing Techniques CRISPR-Cas Systems DNA / genetics Immunoassay

Journal

The Analyst
ISSN: 1364-5528
Titre abrégé: Analyst
Pays: England
ID NLM: 0372652

Informations de publication

Date de publication:
22 Aug 2022
Historique:
pubmed: 4 8 2022
medline: 24 8 2022
entrez: 3 8 2022
Statut: epublish

Résumé

Using the DNA activator of Cas to label the secondary antibody of traditional ELISA, the CRISPR/Cas12a-based fluorescence immunoassay was presented. The sensing strategy ingeniously combines the efficient signal generation of the CRISPR/Cas12a system with antigen-antibody-specific recognition. This work provides an alternative strategy to expand the application of the CRISPR/Cas system for the detection of non-nucleic acid targets.

Identifiants

DOI: 10.1039/d2an01048h PMID: 35920723
pubmed: 35920723
doi: 10.1039/d2an01048h
doi:

Substances chimiques

DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

3833-3837

Auteurs

Xi Li (X)

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China. libaoxin@snnu.edu.cn.

Yuyuan Zhang (Y)

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China. libaoxin@snnu.edu.cn.

Qiang Liu (Q)

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China. libaoxin@snnu.edu.cn.

Yan Jin (Y)

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China. libaoxin@snnu.edu.cn.
ORCID: 0000-0001-8051-9950

Baoxin Li (B)

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China. libaoxin@snnu.edu.cn.
ORCID: 0000-0002-9294-4572

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › CRISPR/Cas12a-based fluorescence immunoassay:...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › CRISPR/Cas12a-based fluorescence immunoassay:...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › CRISPR/Cas12a-based fluorescence immunoassay:...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › CRISPR/Cas12a-based fluorescence immunoassay:...