Unraveling the enzymatic and antibacterial potential of rare halophilic actinomycetes from Algerian hypersaline wetland ecosystems.
Actinobacteria
Actinomyces
/ genetics
Agar
Algeria
Amylases
Anti-Bacterial Agents
/ pharmacology
Asparaginase
/ genetics
Cellulases
/ genetics
Ecosystem
Esterases
/ genetics
Glutaminase
/ genetics
Peptide Hydrolases
/ genetics
Phylogeny
Polygalacturonase
RNA, Ribosomal, 16S
/ genetics
Salts
Soil
Wetlands
Algeria
antibacterial activity
dry salt lakes (Chott and Sebkha)
hydrolytic enzyme
rare halophilic actinomycetes
wetland ecosystems
Journal
Journal of basic microbiology
ISSN: 1521-4028
Titre abrégé: J Basic Microbiol
Pays: Germany
ID NLM: 8503885
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
revised:
18
06
2022
received:
12
02
2022
accepted:
10
07
2022
pubmed:
10
8
2022
medline:
5
10
2022
entrez:
9
8
2022
Statut:
ppublish
Résumé
The study aimed to isolate rare halophilic actinomycetes from hypersaline soils of Algerian inland Wetland Ecosystems "Sebkhas-Chotts" located in arid and hot hyperarid lands with international importance under the Ramsar Convention and to explore their enzyme-producing and antibacterial abilities. The halophilic actinomycetes were selectively isolated using agar-rich media supplemented with 5, 10, and 15% (W/V) of total salts. Thirty-one isolates were obtained and 16S rRNA gene sequencing analysis revealed the presence of members affiliated to rare halophilic actinobacterial genera (Actinopolyspora and Nocardiopsis) accounting for 74.19% (23 isolates out of 31) and 25.8% (8 isolates), respectively. Both phylotypes are alkalitolerant and halophilic thermotolerant actinomycetes displaying significant hydrolytic activities relative to (amylase, asparaginase, cellulase, esterase, glutaminase, inulinase, protease, pectinase, xylanase), and over 96% of tested isolates exhibited all common enzymes, mainly active at 10% of growing salt. In addition, high antibacterial activity was observed against Bacillus cereus, Bacillus subtilis, Micrococcus luteus, and Staphylococcus aureus. The findings showed that saline wetlands ecosystems represent a rich reservoir for the isolation of significant rare halophilic actinomycetes with potential adaptive features and valuable sources for novel bioactive metabolites and biocatalysts of biotechnological interest.
Identifiants
pubmed: 35945171
doi: 10.1002/jobm.202200085
doi:
Substances chimiques
Anti-Bacterial Agents
0
RNA, Ribosomal, 16S
0
Salts
0
Soil
0
Agar
9002-18-0
Esterases
EC 3.1.-
Amylases
EC 3.2.1.-
Cellulases
EC 3.2.1.-
Polygalacturonase
EC 3.2.1.15
Peptide Hydrolases
EC 3.4.-
Asparaginase
EC 3.5.1.1
Glutaminase
EC 3.5.1.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1202-1215Informations de copyright
© 2022 Wiley-VCH GmbH.
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