Evaluating the potential of Pediococcus pentosaceus as a biocontrol agent against tenuazonic acid-producing Alternaria alternata on livestock feeds.
Alternaria alternata
Pediococcus pentosaceus
antifungal effects
grain silage
mycotoxins
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
revised:
01
07
2022
received:
06
04
2022
accepted:
27
07
2022
pubmed:
11
8
2022
medline:
22
10
2022
entrez:
10
8
2022
Statut:
ppublish
Résumé
This study aims to demonstrate the potential of the lactic acid bacteria (LAB) Pediococcus pentosaceus LBM18 against the mycotoxin-producing Alternaria alternata TEF-1A and highlight its application as an effective grain silage inoculant to control mycotoxin contamination. The antifungal properties of Ped. pentosaceus lyophilized (PPL) were assessed by evaluating its effect on A. alternata TEF-1A grown in a corn silage-based medium, which included morphological changes by Scanning Electron Microscopy (SEM) observations, growth rate, conidia production assays, and inhibition of Tenuazonic acid (TeA) production by high-performance liquid chromatography (HPLC-MS/MS) analyses. Furthermore, TeA biosynthesis was monitored for changes at the molecular level by PKS gene expression. The growth and sporulation processes of A. alternata TEF-1A were affected by Ped. pentosaceus LBM18 in a concentration-dependent manner. Moreover, a significant inhibition of TeA production (74.3%) and the transcription level of the PKS gene (42.9%) was observed. Ped. pentosaceus is one of the promising LAB to be applied as an inoculant for corn silage preservation, aiming to inhibit mycotoxigenic fungi growth and their mycotoxin production. Ped. pentosaceus could be used as an inoculant to reduce fungal and mycotoxins contamination in grain silage production.
Substances chimiques
Tenuazonic Acid
610-88-8
Antifungal Agents
0
Mycotoxins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3020-3029Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 312923/2020-1
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 408783/2021-4
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2018/25511-1
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2019/02456-8
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2019/16551-2
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2020/10676-5
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2020/13271-6
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2020/14144-8
Informations de copyright
© 2022 Society for Applied Microbiology.
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