Complete genome sequence of a novel lytic bacteriophage, PLG-II, specific for Lactococcus garvieae serotype II strains that are pathogenic to fish.


Journal

Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870

Informations de publication

Date de publication:
Nov 2022
Historique:
received: 21 03 2022
accepted: 05 07 2022
pubmed: 17 8 2022
medline: 15 10 2022
entrez: 16 8 2022
Statut: ppublish

Résumé

A novel lytic siphophage, PLG-II, which is specific for Lactococcus garvieae serotype II strains that are pathogenic to fish, was isolated from seawater samples collected from Miyazaki Prefecture, Japan. Whole-genome sequencing showed that the PLG-II genome is a 32,271-bp double-stranded DNA molecule, with an average GC content of 37.74%. It contains 69 open reading frames (ORFs), 43 of which currently have no reliable functional annotation for their product, as well as a single tRNA. Comparative genomics analysis suggests that phage PLG-II might represent a novel species in the genus Uwajimavirus.

Identifiants

pubmed: 35972540
doi: 10.1007/s00705-022-05568-7
pii: 10.1007/s00705-022-05568-7
doi:

Substances chimiques

DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2331-2335

Subventions

Organisme : Ministry of Education, Culture, Sports, Science and Technology
ID : 21H02287

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

Références

Ohbayashi K, Oinaka D, Hoai TD et al (2017) PCR-mediated identification of the newly emerging pathogen Lactococcus garvieae serotype II from Seriola quinqueradiata and S. dumerili. Fish Pathol 52:46–49. https://doi.org/10.3147/jsfp.52.46
doi: 10.3147/jsfp.52.46
Nishiki I, Furukawa M, Matui S et al (2011) Epidemiological study on Lactococcus garvieae isolates from fish in Japan. Fish Sci 77:367–373. https://doi.org/10.1007/s12562-011-0332-0
doi: 10.1007/s12562-011-0332-0
Oinaka D, Yoshimura N, Fukuda Y et al (2015) Isolation of Lactococcus garvieae showing no agglutination with anti-KG-phenotype rabbit serum. Fish Pathol 50:37–43. https://doi.org/10.3147/jsfp.50.37
doi: 10.3147/jsfp.50.37
Fukuda Y, Tue Y, Oinaka D et al (2015) Pathogenicity and immunogenicity of non-agglutinating Lactococcus garvieae with anti-KG-phenotype rabbit serum in Seriola spp. Fish Pathol 50:200–206. https://doi.org/10.3147/jsfp.50.200
doi: 10.3147/jsfp.50.200
Shi YZ, Nishiki I, Yanagi S, Yoshida T (2019) Epidemiological study on newly emerging lactococcus garvieae serotype ii isolated from marine fish species in japan. Fish Pathol 54:51–57. https://doi.org/10.3147/jsfp.54.51
doi: 10.3147/jsfp.54.51
Mirzaei MK, Nilsson AS (2015) Isolation of phages for phage therapy: a comparison of spot tests and efficiency of plating analyses for determination of host range and efficacy. PLoS ONE 10:e0118557. https://doi.org/10.1371/JOURNAL.PONE.0118557
doi: 10.1371/JOURNAL.PONE.0118557
Park KH, Matsuoka S, Nakai T, Muroga K (1997) A virulent bacteriophage of Lactococcus garvieae (formerly Enterococcus seriolicida) isolated from yellowtail Seriola quinqueradiata. Dis Aquat Org 29:145–149. https://doi.org/10.3354/dao029145
doi: 10.3354/dao029145
Park KH, Kato H, Nakai T, Muroga K (1998) Phage typing of lactococcus garvieae (formerly enterococcus seriolicida) a pathogen of cultured yellowtail. Fish Sci 64:62–64. https://doi.org/10.2331/fishsci.64.62
doi: 10.2331/fishsci.64.62
Nakai T, Sugimoto R, Park KH et al (1999) Protective effects of bacteriophage on experimental Lactococcus garvieae infection in yellowtail. Dis Aquat Organ 37:33–41. https://doi.org/10.3354/dao037033
doi: 10.3354/dao037033 pubmed: 10439901
Gencay YE, Birk T, Sørensen MCH, Brøndsted L (2017) Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni. Methods Mol Biol 1512:19–28. https://doi.org/10.1007/978-1-4939-6536-6_3
doi: 10.1007/978-1-4939-6536-6_3 pubmed: 27885595
Hoai T, Nishiki I, Yoshida T, Nakai T (2018) Host range and influence of a cell capsule on the phage efficacy of three Lactococcus garvieae lytic phages. Dis Aquat Org 128:81–86
doi: 10.3354/dao03212
Andrews S (2010) FastQC—A quality control tool for high throughput sequence data. accessed on 20th March 2021  http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ 
Bushnell B (2014) BBMap: a fast, accurate, splice-aware aligner. In: Conference 9th annual genomics of energy & environment meeting, Walnut Creek, CA, March 17–20, 2014
Wick RR, Judd LM, Gorrie CL, Holt KE (2017) Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 13:1–22. https://doi.org/10.1371/journal.pcbi.1005595
doi: 10.1371/journal.pcbi.1005595
Altschup SF, Gish W, Miller W et al (1990) Basic local alignment search tool. J Mol Biol 215:403–410
doi: 10.1016/S0022-2836(05)80360-2
Delcher AL, Bratke KA, Powers EC, Salzberg SL (2007) Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics. https://doi.org/10.1093/bioinformatics/btm009
doi: 10.1093/bioinformatics/btm009 pubmed: 18070356 pmcid: 2222658
Besemer J, Borodovsky M (2005) GeneMark: web software for gene finding in prokaryotes, eukaryotes and viruses. Nucleic Acids Res 33:451–454. https://doi.org/10.1093/nar/gki487
doi: 10.1093/nar/gki487
Laslett D, Canback B (2004) ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences. Nucleic Acids Res 32:11–16. https://doi.org/10.1093/nar/gkh152
doi: 10.1093/nar/gkh152 pubmed: 14704338 pmcid: 373265
Lowe TM, Eddy SR (1997) tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 25:955–964. https://doi.org/10.1093/NAR/25.5.955
doi: 10.1093/NAR/25.5.955 pubmed: 9023104 pmcid: 146525
Pope WH, Jacobs-Sera D (2018) Annotation of bacteriophage genome sequences using DNA master: an overview. In: Methods in molecular biology. Humana Press Inc., pp 217–229
Zrelovs N, Dislers A, Kazaks A (2021) Genome characterization of nocturne116, novel lactococcus lactis-infecting phage isolated from moth. Microorg. 9:1540. https://doi.org/10.3390/MICROORGANISMS9071540
doi: 10.3390/MICROORGANISMS9071540
Moraru C, Varsani A, Viruses AK-2020 U (2020) VIRIDIC—a novel tool to calculate the intergenomic similarities of prokaryote-infecting viruses. mdpi.com 12
Sievers F, Wilm A, Dineen D et al (2011) Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol 7:539. https://doi.org/10.1038/MSB.2011.75
doi: 10.1038/MSB.2011.75 pubmed: 21988835 pmcid: 3261699
Nguyen LT, Schmidt HA, Von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 32:268–274. https://doi.org/10.1093/MOLBEV/MSU300
doi: 10.1093/MOLBEV/MSU300 pubmed: 25371430
Kalyaanamoorthy S, Minh BQ, Wong TKF et al (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nat Methods 146(14):587–589. https://doi.org/10.1038/nmeth.4285
doi: 10.1038/nmeth.4285
Minh BQ, Nguyen MAT, Von Haeseler A (2013) Ultrafast approximation for phylogenetic bootstrap. Mol Biol Evol 30:1188–1195. https://doi.org/10.1093/MOLBEV/MST024
doi: 10.1093/MOLBEV/MST024 pubmed: 23418397 pmcid: 3670741
Sullivan MJ, Petty NK, Beatson SA (2011) Easyfig: a genome comparison visualizer. Bioinformatics 27:1009–1010. https://doi.org/10.1093/BIOINFORMATICS/BTR039
doi: 10.1093/BIOINFORMATICS/BTR039 pubmed: 21278367 pmcid: 3065679
Turner D, Kropinski AM, Adriaenssens EM (2021) A roadmap for genome-based phage taxonomy. Viruses 13:506. https://doi.org/10.3390/V13030506
doi: 10.3390/V13030506 pubmed: 33803862 pmcid: 8003253

Auteurs

Muhammad Akmal (M)

Faculty of Agriculture, University of Miyazaki, Gauken Kibanadai Nishi 1-1, Miyazaki, 889-2192, Japan. nb21005@student.miyazaki-u.ac.jp.
Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan. nb21005@student.miyazaki-u.ac.jp.

Issei Nishiki (I)

Faculty of Agriculture, University of Miyazaki, Gauken Kibanadai Nishi 1-1, Miyazaki, 889-2192, Japan.

Nikita Zrelovs (N)

Latvian Biomedical Research and Study Centre, Ratsupites 1 k1, Riga, 1067, Latvia.

Terutoyo Yoshida (T)

Faculty of Agriculture, University of Miyazaki, Gauken Kibanadai Nishi 1-1, Miyazaki, 889-2192, Japan.

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