Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine-Wave Illuminated Reversibly Photoactivatable Labels.
fluorescence imaging
harmonics
periodic forcing
resonance
reversibly photoactivatable label
Journal
Chemphyschem : a European journal of chemical physics and physical chemistry
ISSN: 1439-7641
Titre abrégé: Chemphyschem
Pays: Germany
ID NLM: 100954211
Informations de publication
Date de publication:
05 12 2022
05 12 2022
Historique:
revised:
15
08
2022
received:
29
04
2022
pubmed:
18
8
2022
medline:
15
12
2022
entrez:
17
8
2022
Statut:
ppublish
Résumé
We introduce HIGHLIGHT as a simple and general strategy to selectively image a reversibly photoactivatable fluorescent label associated with a given kinetics. The label is submitted to sine-wave illumination of large amplitude, which generates oscillations of its concentration and fluorescence at higher harmonic frequencies. For singularizing a label, HIGHLIGHT uses specific frequencies and mean light intensities associated with resonances of the amplitudes of concentration and fluorescence oscillations at harmonic frequencies. Several non-redundant resonant observables are simultaneously retrieved from a single experiment with phase-sensitive detection. HIGHLIGHT is used for selective imaging of four spectrally similar fluorescent proteins that had not been discriminated so far. Moreover, labels out of targeted locations can be discarded in an inhomogeneous spatial profile of illumination. HIGHLIGHT opens roads for simplified optical setups at reduced cost and easier maintenance.
Identifiants
pubmed: 35976176
doi: 10.1002/cphc.202200295
pmc: PMC10087976
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202200295Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-10-INBS-04
Organisme : Agence Nationale de la Recherche
ID : ANR- 11-EQPX-0029
Organisme : Agence Nationale de la Recherche
ID : ANR-10-IDEX-0001-02 PSL
Organisme : Agence Nationale de la Recherche
ID : ANR-10-LABX-31
Organisme : Agence Nationale de la Recherche
ID : ANR-19-CE11-0005
Organisme : Agence Nationale de la Recherche
ID : ANR-19- CE29-0003-01
Organisme : Fondation de la recherche médicale
ID : FRM DEI201512440
Organisme : Sorbonne Université
ID : Emergence 2019-2020, LIGHTNING
Organisme : DIM Ile de France
ID : DREAM
Organisme : EIC Path Finder OPEN DREAM
ID : 101046451
Informations de copyright
© 2022 The Authors. ChemPhysChem published by Wiley-VCH GmbH.
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