Reticulons 3 and 6 interact with viral movement proteins.
FRET-FLIM
endoplasmic reticulum
plasmodesmata
protein-protein interaction
reticulon
viral movement protein
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
12 2022
12 2022
Historique:
revised:
05
08
2022
received:
11
03
2022
accepted:
05
08
2022
pubmed:
21
8
2022
medline:
11
11
2022
entrez:
20
8
2022
Statut:
ppublish
Résumé
Plant reticulon (RTN) proteins are capable of constricting membranes and are vital for creating and maintaining tubules in the endoplasmic reticulum (ER), making them prime candidates for the formation of the desmotubule in plasmodesmata (PD). RTN3 and RTN6 have previously been detected in an Arabidopsis PD proteome and have been shown to be present in primary PD at cytokinesis. It has been suggested that RTN proteins form protein complexes with proteins in the PD plasma membrane and desmotubule to stabilize the desmotubule constriction and regulate PD aperture. Viral movement proteins (vMPs) enable the transport of viruses through PD and can be ER-integral membrane proteins or interact with the ER. Some vMPs can themselves constrict ER membranes or localize to RTN-containing tubules; RTN proteins and vMPs could be functionally linked or potentially interact. Here we show that different vMPs are capable of interacting with RTN3 and RTN6 in a membrane yeast two-hybrid assay, coimmunoprecipitation, and Förster resonance energy transfer measured by donor excited-state fluorescence lifetime imaging microscopy. Furthermore, coexpression of the vMP CMV-3a and RTN3 results in either the vMP or the RTN changing subcellular localization and reduces the ability of CMV-3a to open PD, further indicating interactions between the two proteins.
Identifiants
pubmed: 35987858
doi: 10.1111/mpp.13261
pmc: PMC9644274
doi:
Substances chimiques
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1807-1814Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M007200/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/J004987/1
Pays : United Kingdom
Informations de copyright
© 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.
Références
Plant Cell. 2009 Dec;21(12):3937-49
pubmed: 20040535
J Biol Chem. 2002 Jun 28;277(26):23447-52
pubmed: 11976343
PLoS Pathog. 2016 Feb 10;12(2):e1005443
pubmed: 26863622
J Gen Virol. 2005 Apr;86(Pt 4):1223-1228
pubmed: 15784916
Plant Cell. 1999 Apr;11(4):615-28
pubmed: 10213782
Plant J. 2010 Nov;64(3):411-8
pubmed: 20969742
Trends Plant Sci. 2009 Feb;14(2):92-9
pubmed: 19162525
Plant Physiol. 2010 Oct;154(2):458-62
pubmed: 20921163
Plant Physiol. 2012 Mar;158(3):1359-70
pubmed: 22253256
Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2491-6
pubmed: 20133785
FEBS Lett. 2014 May 2;588(9):1699-705
pubmed: 24657438
Plant J. 2003 May;34(3):269-81
pubmed: 12713534
New Phytol. 2021 Jan;229(2):1052-1066
pubmed: 32866987
Traffic. 2010 Jul 1;11(7):912-30
pubmed: 20374554
Virology. 2003 Apr 25;309(1):135-51
pubmed: 12726734
Science. 2017 May 12;356(6338):617-624
pubmed: 28495747
Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11169-74
pubmed: 9736708
Curr Opin Microbiol. 2012 Aug;15(4):519-24
pubmed: 22621853
J Virol. 2002 Jun;76(12):6293-301
pubmed: 12021362
Methods Mol Biol. 2018;1691:159-166
pubmed: 29043676
Plant Cell. 2010 Apr;22(4):1333-43
pubmed: 20424177
Curr Biol. 2014 Jun 16;24(12):1397-1405
pubmed: 24909329
Traffic. 2008 Jan;9(1):94-102
pubmed: 17980018
Plant Physiol. 1996 Apr;110(4):1055-61
pubmed: 8934621
BMC Cell Biol. 2005 Apr 26;6(1):22
pubmed: 15854225
Protoplasma. 2011 Jan;248(1):39-60
pubmed: 20938697
Methods Mol Biol. 2014;1209:81-95
pubmed: 25117276
Biochem J. 2009 Sep 25;423(2):145-55
pubmed: 19772494
Curr Biol. 2015 Aug 3;25(15):2018-25
pubmed: 26166780
PLoS One. 2011 Apr 20;6(4):e18880
pubmed: 21533090
Front Plant Sci. 2020 Jul 03;11:862
pubmed: 32719692
Plant Physiol. 2005 Jan;137(1):104-16
pubmed: 15618420
Plant Physiol. 2015 Nov;169(3):1933-45
pubmed: 26353761
J Cell Biol. 1999 Nov 29;147(5):945-58
pubmed: 10579716
Curr Opin Plant Biol. 2008 Dec;11(6):672-9
pubmed: 18824401
J Gen Virol. 2006 Aug;87(Pt 8):2403-2411
pubmed: 16847137
Mol Plant Pathol. 2022 Dec;23(12):1807-1814
pubmed: 35987858
Virology. 1997 Oct 27;237(2):237-48
pubmed: 9356336
Plant Physiol. 2015 May;168(1):132-43
pubmed: 25792253
Plant J. 2010 Mar;61(6):1092-106
pubmed: 20409280
J Exp Bot. 2016 Jul;67(14):4195-207
pubmed: 27208541
J Microsc. 2012 Aug;247(2):119-36
pubmed: 22621335
Front Plant Sci. 2014 Nov 06;5:584
pubmed: 25414709
PLoS Pathog. 2014 Oct 16;10(10):e1004388
pubmed: 25329172
Eur J Cell Biol. 1999 May;78(5):297-304
pubmed: 10384980
PLoS Pathog. 2016 Oct 27;12(10):e1005912
pubmed: 27788266
Curr Opin Plant Biol. 2012 Aug;15(4):367-74
pubmed: 22664220
Methods Mol Biol. 2018;1691:191-203
pubmed: 29043679
J Cell Biol. 2013 Jun 24;201(7):981-95
pubmed: 23798728
New Phytol. 2020 May;226(3):798-808
pubmed: 31869440
Plant Cell. 2005 Jan;17(1):164-81
pubmed: 15608333
Virology. 2003 Jul 20;312(1):35-48
pubmed: 12890619
J Virol. 2014 Mar;88(5):3016-26
pubmed: 24371064
J Microsc. 2020 Nov;280(2):122-133
pubmed: 32426862
Plant Physiol. 2015 Aug;168(4):1563-72
pubmed: 26084919
Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16291-6
pubmed: 20805477
J Integr Plant Biol. 2015 Jan;57(1):50-62
pubmed: 25319240
Mol Plant. 2008 Nov;1(6):910-24
pubmed: 19825592