Microbial degradation of low density polyethylene by Exiguobacterium sp. strain LM-IK2 isolated from plastic dumped soil.
Biodegradation
Exiguobacterium
Low density polyethylene
Percent weight loss
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
22 Aug 2022
22 Aug 2022
Historique:
received:
25
04
2022
accepted:
13
08
2022
entrez:
21
8
2022
pubmed:
22
8
2022
medline:
24
8
2022
Statut:
epublish
Résumé
Low-Density Polyethylene (LDPE) is one of the significant environmental pollutants as it is resistant to natural degradation. In this study, we reported the LDPE-degrading bacterial strain i.e., Exiguobacterium sp. strain LM-IK2 isolated from plastic dumped soil which shows potential degradation capability. The percent weight loss of LDPE was calculated as - 5.70 ± 0.7 after 90 days of incubation in a carbon-free MSM medium. The Field Emission Scanning Electron Microscopy (FE-SEM) analysis shows that LDPE films show slight surface disruption after treatment with bacteria. The Fourier Transform Infrared Spectroscopy (FTIR) revealed the chemical changes in LDPE films e.g., formation and reduction of typical carbonyl peaks after incubation with bacteria. The X-Ray Diffraction (XRD) analysis displayed an increase in percent crystallinity, with a slight change in total carbon content. Genetic analysis showed the presence of Laccase (167 bp) and Alkane Hydroxylase (330 bp) genes that are responsible for LDPE degradation. Thus, Exiguobacterium sp. strain LM-IK2 has the potential to degrade LDPE and could be further explored to improve its efficiency in the bioremediation of LDPE.
Identifiants
pubmed: 35989357
doi: 10.1007/s11274-022-03389-z
pii: 10.1007/s11274-022-03389-z
doi:
Substances chimiques
Plastics
0
Soil
0
Polyethylene
9002-88-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
197Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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