Reinforced Hyaluronic Acid-Based Matrices Promote 3D Neuronal Network Formation.
3D model systems
Ca2+-Imaging
astrocytes
cortical neurons
hyaluronic acid
melt electrowriting
Journal
Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
received:
02
08
2022
pubmed:
23
8
2022
medline:
4
11
2022
entrez:
22
8
2022
Statut:
ppublish
Résumé
3D neuronal cultures attempt to better replicate the in vivo environment to study neurological/neurodegenerative diseases compared to 2D models. A challenge to establish 3D neuron culture models is the low elastic modulus (30-500 Pa) of the native brain. Here, an ultra-soft matrix based on thiolated hyaluronic acid (HA-SH) reinforced with a microfiber frame is formulated and used. Hyaluronic acid represents an essential component of the brain extracellular matrix (ECM). Box-shaped frames with a microfiber spacing of 200 µm composed of 10-layers of poly(ɛ-caprolactone) (PCL) microfibers (9.7 ± 0.2 µm) made via melt electrowriting (MEW) are used to reinforce the HA-SH matrix which has an elastic modulus of 95 Pa. The neuronal viability is low in pure HA-SH matrix, however, when astrocytes are pre-seeded below this reinforced construct, they significantly support neuronal survival, network formation quantified by neurite length, and neuronal firing shown by Ca
Identifiants
pubmed: 35993391
doi: 10.1002/adhm.202201826
doi:
Substances chimiques
Hyaluronic Acid
9004-61-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2201826Informations de copyright
© 2022 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.
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