Multivalent Ligand-Nanoparticle Conjugates Amplify Reactive Oxygen Species Second Messenger Generation and Enhance Epidermal Growth Factor Receptor Phosphorylation.
Journal
Bioconjugate chemistry
ISSN: 1520-4812
Titre abrégé: Bioconjug Chem
Pays: United States
ID NLM: 9010319
Informations de publication
Date de publication:
21 Sep 2022
21 Sep 2022
Historique:
pubmed:
23
8
2022
medline:
24
9
2022
entrez:
22
8
2022
Statut:
ppublish
Résumé
The epidermal growth factor (EGF) receptor (EGFR) is heterogeneously distributed on the cellular surface and enriched in clusters with diameters of tens of nanometers. Multivalent presentation of EGF ligand on nanoparticles (NPs) provides an approach for controlling and amplifying the local activation of EGFR in these clusters. Reactive oxygen species (ROS) have been indicated to play a role in the regulation of EGFR activation as second messengers, but the effect of nanoconjugation on EGF-mediated ROS formation and ROS-induced EGFR activation is not well established. The goal of this manuscript is to characterize the multivalent enhancement of EGF-induced ROS formation and to test its effect on EGFR phosphorylation in breast cancer cell models using gold (Au) NPs with a diameter of 81 ± 1 nm functionalized with two different EGF ligand densities (12 ± 7 EGF/NP (NP-EGF
Identifiants
pubmed: 35993676
doi: 10.1021/acs.bioconjchem.2c00335
pmc: PMC9815836
mid: NIHMS1852807
doi:
Substances chimiques
Ligands
0
Reactive Oxygen Species
0
NADP
53-59-8
Epidermal Growth Factor
62229-50-9
Gold
7440-57-5
Oxidoreductases
EC 1.-
ErbB Receptors
EC 2.7.10.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1716-1728Subventions
Organisme : NCI NIH HHS
ID : R01 CA138509
Pays : United States
Références
Chem Commun (Camb). 2016 Dec 22;53(2):317-320
pubmed: 27853769
Small. 2017 Jul;13(26):
pubmed: 28544579
Nanomedicine. 2011 Apr;7(2):201-9
pubmed: 20816874
Angew Chem Int Ed Engl. 2001 Jun 1;40(11):2004-2021
pubmed: 11433435
Commun Biol. 2021 May 19;4(1):597
pubmed: 34011948
J Cell Biol. 2008 Jun 30;181(7):1129-39
pubmed: 18573911
J Control Release. 2014 Nov 28;194:20-7
pubmed: 25128717
Free Radic Biol Med. 2015 Dec;89:47-53
pubmed: 26169725
J Biol Chem. 2010 Jan 29;285(5):3145-56
pubmed: 19959837
J Am Chem Soc. 2010 Aug 18;132(32):11306-13
pubmed: 20698697
ACS Nano. 2020 Jan 28;14(1):372-383
pubmed: 31899613
Nat Biomed Eng. 2018 Feb;2(2):95-103
pubmed: 29955439
J Biol Chem. 1998 Jun 19;273(25):15366-72
pubmed: 9624118
Nat Chem Biol. 2011 Jul 18;7(8):504-11
pubmed: 21769097
J Biol Chem. 2002 Sep 20;277(38):35671-81
pubmed: 12105206
J Biol Chem. 2004 Jul 9;279(28):28970-8
pubmed: 15123705
Am J Cancer Res. 2016 Aug 01;6(8):1609-23
pubmed: 27648353
J Cell Biol. 2011 Jul 11;194(1):7-15
pubmed: 21746850
FEBS Lett. 1995 Jan 3;357(2):161-4
pubmed: 7805884
ACS Nano. 2018 Aug 28;12(8):8423-8435
pubmed: 30016073
Cell. 2010 Jun 25;141(7):1117-34
pubmed: 20602996
Chem Commun (Camb). 2011 Aug 14;47(30):8620-2
pubmed: 21720651
Pharmacol Res. 2014 Jan;79:34-74
pubmed: 24269963
Chem Soc Rev. 2015 Aug 7;44(15):5495-551
pubmed: 26000775
Cell. 2006 Jun 16;125(6):1137-49
pubmed: 16777603
Am J Physiol Cell Physiol. 2004 Aug;287(2):C246-56
pubmed: 15238356
Trends Cell Biol. 2014 Jan;24(1):26-34
pubmed: 24295852
Nat Chem Biol. 2007 May;3(5):263-7
pubmed: 17401379
Curr Biol. 2014 May 19;24(10):R453-62
pubmed: 24845678
Cell Res. 2014 Aug;24(8):959-76
pubmed: 25001389
Sci Rep. 2013;3:2534
pubmed: 23982586
Cell Chem Biol. 2016 Jul 21;23(7):837-848
pubmed: 27427230
Nanoscale. 2018 Apr 5;10(14):6712-6723
pubmed: 29589623
Chem Biol. 2007 Jan;14(1):107-15
pubmed: 17254956
J Mass Spectrom. 2009 May;44(5):694-706
pubmed: 19132714
J Am Chem Soc. 2002 Dec 18;124(50):14922-33
pubmed: 12475334
Biomacromolecules. 2016 Mar 14;17(3):710-22
pubmed: 26771518
Exp Mol Med. 2016 Nov 4;48(11):e269
pubmed: 27811934
ACS Nano. 2022 Mar 22;16(3):3785-3796
pubmed: 35274534
Anal Chem. 2010 Nov 1;82(21):9082-9
pubmed: 20942402
Bioconjug Chem. 2022 Mar 16;33(3):452-462
pubmed: 35167278
Onco Targets Ther. 2019 Sep 20;12:7749-7756
pubmed: 31571924
Nat Commun. 2016 Dec 19;7:13818
pubmed: 27991503
ACS Chem Biol. 2013 Sep 20;8(9):2063-71
pubmed: 23855478
Cell. 2002 Sep 20;110(6):669-72
pubmed: 12297041
Chem Commun (Camb). 2003 Dec 7;(23):2920-1
pubmed: 14680241
J Phys Chem B. 2017 Apr 13;121(14):2942-2951
pubmed: 28334537
Growth Factors. 2008 Dec;26(6):316-24
pubmed: 18937111
Cancers (Basel). 2017 May 17;9(5):
pubmed: 28513565
Nanoscale. 2016 Dec 28;8(48):20037-20047
pubmed: 27883139
Free Radic Res. 2010 May;44(5):479-96
pubmed: 20370557
Mol Cell Biol. 2010 Aug;30(15):3795-804
pubmed: 20516214
Biochem J. 2009 Jan 1;417(1):1-13
pubmed: 19061483
Nat Nanotechnol. 2013 Nov;8(11):831-8
pubmed: 24141540
Nat Cell Biol. 2003 May;5(5):447-53
pubmed: 12717446
Mol Cell Biol. 2000 Oct;20(19):7311-8
pubmed: 10982848
Mol Aspects Med. 2009 Feb-Apr;30(1-2):42-59
pubmed: 18601945
Nat Chem Biol. 2008 May;4(5):278-86
pubmed: 18421291
Science. 2000 Nov 24;290(5496):1567-70
pubmed: 11090353
ACS Appl Mater Interfaces. 2019 Jul 31;11(30):26722-26730
pubmed: 31276364
Small. 2014 Oct 29;10(20):4182-92
pubmed: 24990622
Am J Respir Cell Mol Biol. 1998 Nov;19(5):786-98
pubmed: 9806743
ACS Appl Mater Interfaces. 2019 Jan 9;11(1):1311-1320
pubmed: 30521749
Elife. 2016 Mar 28;5:
pubmed: 27017828
Physiol Rev. 2002 Jan;82(1):47-95
pubmed: 11773609
J Am Chem Soc. 2019 Feb 13;141(6):2577-2588
pubmed: 30676018
Cancer Cell. 2003 Jun;3(6):519-23
pubmed: 12842080
Nanoscale. 2015 Feb 14;7(6):2511-9
pubmed: 25569174
Nat Rev Mol Cell Biol. 2007 Oct;8(10):813-24
pubmed: 17848967
Biomater Sci. 2014 Feb 1;2(2):156-166
pubmed: 24683470
Science. 1995 Oct 13;270(5234):296-9
pubmed: 7569979
Mol Biosyst. 2013 Jul;9(7):1849-63
pubmed: 23629589
Nat Chem Biol. 2011 Dec 11;8(1):57-64
pubmed: 22158416
ACS Nano. 2018 Oct 23;12(10):10473-10485
pubmed: 30289688
Nanomedicine. 2013 Feb;9(2):194-201
pubmed: 22687896
Mol Cell. 2002 Feb;9(2):387-99
pubmed: 11864611
J Biol Chem. 1997 Jan 3;272(1):217-21
pubmed: 8995250
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16163-8
pubmed: 19805274
Nat Rev Immunol. 2004 Mar;4(3):181-9
pubmed: 15039755
J Phys Chem B. 2019 Jul 5;123(26):5494-5505
pubmed: 31244098
Bioconjug Chem. 2017 Jun 21;28(6):1649-1657
pubmed: 28398751
Biochem Biophys Res Commun. 2005 Aug 19;334(1):38-42
pubmed: 15982634
Biochemistry. 1987 Mar 10;26(5):1434-42
pubmed: 3494472
J Biol Chem. 2010 Dec 10;285(50):39481-9
pubmed: 20940297
Nanoscale. 2019 Mar 14;11(11):5005-5013
pubmed: 30839969
Angew Chem Int Ed Engl. 2010 Sep 24;49(40):7266-70
pubmed: 20740515
Mol Cell Biol. 2004 May;24(10):4384-94
pubmed: 15121857