Different membrane order measurement techniques are not mutually consistent.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
21 03 2023
21 03 2023
Historique:
received:
07
06
2022
revised:
10
08
2022
accepted:
19
08
2022
pmc-release:
21
03
2024
pubmed:
26
8
2022
medline:
25
3
2023
entrez:
25
8
2022
Statut:
ppublish
Résumé
"Membrane order" is a term commonly used to describe the elastic and mechanical properties of the lipid bilayer, though its exact meaning is somewhat context- and method dependent. These mechanical properties of the membrane control many cellular functions and are measured using various biophysical techniques. Here, we ask if the results obtained from various techniques are mutually consistent. Such consistency cannot be assumed a priori because these techniques probe different spatial locations and different spatial and temporal scales. We evaluate the change of membrane order induced by serotonin using nine different techniques in lipid bilayers of three different compositions. Serotonin is an important neurotransmitter present at 100s of mM concentrations in neurotransmitter vesicles, and therefore its interaction with the lipid bilayer is biologically relevant. Our measurement tools include fluorescence of lipophilic dyes (Nile Red, Laurdan, TMA-DPH, DPH), whose properties are a function of membrane order; atomic force spectroscopy, which provides a measure of the force required to indent the lipid bilayer;
Identifiants
pubmed: 36004780
pii: S0006-3495(22)00689-0
doi: 10.1016/j.bpj.2022.08.029
pmc: PMC10111216
pii:
doi:
Substances chimiques
Lipid Bilayers
0
Phosphatidylcholines
0
Serotonin
333DO1RDJY
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
964-972Informations de copyright
Copyright © 2022 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
Proc Natl Acad Sci U S A. 2012 May 8;109(19):7314-9
pubmed: 22529342
J Cell Biol. 2006 Aug 28;174(5):725-34
pubmed: 16943184
J Biol Chem. 2020 Feb 14;295(7):2136-2147
pubmed: 31796629
Biochim Biophys Acta. 2000 Dec 20;1509(1-2):131-47
pubmed: 11118525
Biophys J. 2020 Mar 10;118(5):1044-1057
pubmed: 32032504
Methods. 2002 Aug;27(4):374-87
pubmed: 12217654
Biochemistry. 1998 Dec 8;37(49):17299-308
pubmed: 9860844
Cell Cycle. 2018;17(13):1649-1666
pubmed: 29963969
Commun Biol. 2019 Sep 13;2:337
pubmed: 31531398
Biochim Biophys Acta. 1979 Aug 20;559(2-3):153-208
pubmed: 383152
Phys Chem Chem Phys. 2013 Feb 14;15(6):1976-89
pubmed: 23258433
Biochim Biophys Acta. 1977 Mar 1;465(2):260-74
pubmed: 16250339
Nat Rev Mol Cell Biol. 2020 Apr;21(4):204-224
pubmed: 32071438
Methods Mol Biol. 2014;1076:617-34
pubmed: 24108647
Biochim Biophys Acta Biomembr. 2019 Dec 1;1861(12):183053
pubmed: 31472104
Biochimie. 2022 Dec;203:40-50
pubmed: 35447219
Chem Rev. 2019 May 8;119(9):5849-5880
pubmed: 30747526
Biochim Biophys Acta Biomembr. 2018 Aug;1860(8):1571-1579
pubmed: 29806993
Biochem Biophys Res Commun. 2006 Sep 1;347(3):838-41
pubmed: 16857174
Neurosci Biobehav Rev. 2022 Aug;139:104729
pubmed: 35691469
Front Cell Dev Biol. 2020 Nov 30;8:601145
pubmed: 33330494
Chem Rev. 2017 Oct 11;117(19):12087-12132
pubmed: 28906107
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Feb 15;191:104-110
pubmed: 28992460
Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16645-50
pubmed: 19805351
Chemistry. 2021 May 12;27(27):7533-7541
pubmed: 33502812
Molecules. 2020 Jul 28;25(15):
pubmed: 32731549
Biophys J. 2014 Nov 18;107(10):2274-86
pubmed: 25418296
Mol Neurobiol. 2020 Feb;57(2):910-925
pubmed: 31595461
Biophys Chem. 2020 Oct;265:106431
pubmed: 32707475
Biochim Biophys Acta. 1983 Mar 21;737(1):117-71
pubmed: 6337629
Nat Commun. 2022 Jan 10;13(1):108
pubmed: 35013165
Eur J Clin Invest. 2021 May;51(5):e13455
pubmed: 33210748
Nat Commun. 2020 Nov 9;11(1):5661
pubmed: 33168829
Front Physiol. 2020 Oct 23;11:578868
pubmed: 33192582
Langmuir. 2014 Jul 15;30(27):8160-6
pubmed: 24905799
J Am Chem Soc. 2013 Feb 13;135(6):2164-71
pubmed: 23311719
J Am Chem Soc. 2010 Apr 7;132(13):4907-16
pubmed: 20225874
J Neurochem. 2005 Dec;95(5):1217-26
pubmed: 16269014
Nat Commun. 2020 Apr 9;11(1):1763
pubmed: 32273501
Front Neural Circuits. 2021 Jun 14;15:661899
pubmed: 34194302
J Am Chem Soc. 2017 Aug 16;139(32):10944-10947
pubmed: 28774176
Biophys Chem. 2021 Jun;273:106587
pubmed: 33865153
Chem Rev. 2019 May 8;119(9):6086-6161
pubmed: 30978005
Langmuir. 2020 May 19;36(19):5134-5144
pubmed: 32338922
Int J Mol Sci. 2020 Apr 10;21(7):
pubmed: 32290157
Biophys J. 1991 Jul;60(1):179-89
pubmed: 1883937
Steroids. 2020 Aug;160:108641
pubmed: 32289327
Langmuir. 2017 Apr 4;33(13):3324-3332
pubmed: 28267920
Neuroscience. 2011 Jan 13;172:118-28
pubmed: 20969929
ACS Chem Biol. 2020 Mar 20;15(3):740-750
pubmed: 32078292
Elife. 2020 Jul 14;9:
pubmed: 32662773
J Biol Chem. 2009 Aug 14;284(33):22238-22247
pubmed: 19553682
Biochemistry. 1998 Jun 2;37(22):8180-90
pubmed: 9609714
Toxins (Basel). 2020 Nov 08;12(11):
pubmed: 33171598