Quantification of CGRP-immunoreactive myenteric neurons in mouse colon.
Journal
The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
revised:
08
08
2022
received:
12
06
2022
accepted:
17
08
2022
pubmed:
1
9
2022
medline:
22
10
2022
entrez:
31
8
2022
Statut:
ppublish
Résumé
Quantitative data of biological systems provide valuable baseline information for understanding pathology, experimental perturbations, and computational modeling. In mouse colon, calcitonin gene-related peptide (CGRP) is expressed by myenteric neurons with multiaxonal (Dogiel type II) morphology, characteristic of intrinsic primary afferent neurons (IPANs). Analogous neurons in other species and gut regions represent 5-35% of myenteric neurons. We aimed to quantify proportions of CGRP-immunopositive (CGRP+) myenteric neurons. Colchicine-treated wholemount preparations of proximal, mid, and distal colon were labeled for HuC/D, CGRP, nitric oxide synthase (NOS), and peripherin (Per). The pan-neuronal markers (Hu+/Per+) co-labeled 94% of neurons. Hu+/Per- neurons comprised ∼6%, but Hu-/Per+ cells were rare. Thus, quantification was based on Hu+ myenteric neurons (8576 total; 1225 ± 239 per animal, n = 7). CGRP+ cell bodies were significantly larger than the average of all Hu+ neurons (329 ± 13 vs. 261 ± 12 μm
Substances chimiques
Calcitonin Gene-Related Peptide
JHB2QIZ69Z
Peripherins
0
Nitric Oxide Synthase
EC 1.14.13.39
Colchicine
SML2Y3J35T
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3209-3225Subventions
Organisme : Australian Research Council
ID : DP190103628
Organisme : National Health and Medical Research Council
ID : 1127140
Organisme : National Health and Medical Research Council
ID : 1156416
Informations de copyright
© 2022 Wiley Periodicals LLC.
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