Transcriptomic and methylation analysis of susceptible and tolerant grapevine genotypes following Plasmopara viticola infection.
Journal
Physiologia plantarum
ISSN: 1399-3054
Titre abrégé: Physiol Plant
Pays: Denmark
ID NLM: 1256322
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
revised:
05
08
2022
received:
31
05
2022
accepted:
29
08
2022
pubmed:
3
9
2022
medline:
27
10
2022
entrez:
2
9
2022
Statut:
ppublish
Résumé
Downy mildew, caused by the biotrophic oomycete Plasmopara viticola, is one of the most economically significant grapevine diseases worldwide. Current strategies to cope with this threat rely on the massive use of chemical compounds during each cultivation season. The economic costs and negative environmental impact associated with these applications increased the urge to search for sustainable strategies of disease control. Improved knowledge of plant mechanisms to counteract pathogen infection may allow the development of alternative strategies for plant protection. Epigenetic regulation, in particular DNA methylation, is emerging as a key factor in the context of plant-pathogen interactions associated with the expression modulation of defence genes. To improve our understanding of the genetic and epigenetic mechanisms underpinning grapevine response to P. viticola, we studied the modulation of both 5-mC methylation and gene expression at 6 and 24 h post-infection (hpi). Leaves of two table grape genotypes (Vitis vinifera), selected by breeding activities for their contrasting level of susceptibility to the pathogen, were analysed. Following pathogen infection, we found variations in the 5-mC methylation level and the gene expression profile. The results indicate a genotype-specific response to pathogen infection. The tolerant genotype (N23/018) at 6 hpi exhibits a lower methylation level compared to the susceptible one (N20/020), and it shows an early modulation (at 6 hpi) of defence and epigenetic-related genes during P. viticola infection. These data suggest that the timing of response is an important mechanism to efficiently counteract the pathogen attack.
Identifiants
pubmed: 36053855
doi: 10.1111/ppl.13771
pmc: PMC9826190
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13771Subventions
Organisme : Fundação Ciência e Tecnologia
ID : FCT/MCTES/PIDDAC
Organisme : Fundação Ciência e Tecnologia
ID : IF/00819/2015
Organisme : Fundação Ciência e Tecnologia
ID : PD/BD/142909/2018
Organisme : Fundação Ciência e Tecnologia
ID : UIDB/00006/2020
Organisme : Fundação Ciência e Tecnologia
ID : UIDP/04046/2020
Organisme : International Organisation of Vine and Wine through the 2018 OIV Research grant program (Project: Identification of pathogen related epigenetic marks in grapevine)
Organisme : Partnership for research and innovation in the Mediterranean area (PRIMA) by Project "REVINE - Regenerative agricultural approaches to improve ecosystem services in Mediterranean vineyards" (Italian MUR DM no. 1966/2021)
ID : 20114-2
Organisme : Partnership for research and innovation in the Mediterranean area (PRIMA) by Project "REVINE - Regenerative agricultural approaches to improve ecosystem services in Mediterranean vineyards" (Italian MUR DM no. 1966/2021)
ID : PRIMA/0011/2020
Informations de copyright
© 2022 The Authors. Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society.
Références
Int J Mol Sci. 2020 Mar 10;21(5):
pubmed: 32164313
Bioinformatics. 2009 Apr 15;25(8):1091-3
pubmed: 19237447
Mol Biol Rep. 2020 Jul;47(7):5511-5522
pubmed: 32562176
Proc Natl Acad Sci U S A. 2012 Aug 7;109(32):E2183-91
pubmed: 22733782
Plant Physiol Biochem. 2019 Apr;137:1-13
pubmed: 30710794
PLoS Pathog. 2014 Apr 03;10(4):e1004030
pubmed: 24699527
Front Plant Sci. 2017 Oct 30;8:1860
pubmed: 29163587
Mol Plant Pathol. 2012 May;13(4):388-98
pubmed: 22023111
Cell Host Microbe. 2014 Dec 10;16(6):787-94
pubmed: 25455564
Front Plant Sci. 2017 Apr 19;8:587
pubmed: 28469630
PLoS One. 2018 Feb 21;13(2):e0192170
pubmed: 29466369
Int J Mol Sci. 2020 Feb 21;21(4):
pubmed: 32098241
Front Plant Sci. 2019 Jul 24;10:951
pubmed: 31396252
Front Plant Sci. 2021 Jun 04;12:692328
pubmed: 34149790
Front Plant Sci. 2015 Sep 02;6:675
pubmed: 26388882
Trends Plant Sci. 2017 Jul;22(7):610-623
pubmed: 28587758
Phytopathology. 2009 Aug;99(8):930-42
pubmed: 19594312
Plant Cell Physiol. 2018 Nov 1;59(11):2169-2187
pubmed: 30169685
BMC Plant Biol. 2019 Aug 6;19(1):343
pubmed: 31387524
PLoS Genet. 2011 Dec;7(12):e1002434
pubmed: 22242006
Front Plant Sci. 2016 Aug 11;7:1201
pubmed: 27563304
New Phytol. 2020 Jul;227(2):545-558
pubmed: 32162327
Front Plant Sci. 2022 Jan 03;12:795274
pubmed: 35046981
Mol Plant. 2016 Jul 6;9(7):1051-65
pubmed: 27131447
Plant Physiol. 2017 Jun;174(2):1067-1081
pubmed: 28455401
New Phytol. 2022 Jan;233(1):66-83
pubmed: 34455592
EMBO J. 2020 Oct 1;39(19):e105802
pubmed: 32865261
Annu Rev Phytopathol. 2016 Aug 4;54:579-603
pubmed: 27491436
Genome Biol. 2017 Jul 6;18(1):132
pubmed: 28683755
Front Plant Sci. 2018 Mar 19;9:355
pubmed: 29616066
Front Plant Sci. 2019 May 31;10:650
pubmed: 31214209
Sci Rep. 2019 Feb 26;9(1):2809
pubmed: 30809001
New Phytol. 2019 Jan;221(2):731-737
pubmed: 30156271
PLoS Genet. 2020 Oct 8;16(10):e1009034
pubmed: 33031395
Mol Plant Microbe Interact. 2016 Mar;29(3):187-96
pubmed: 26524162
J Proteomics. 2017 Jan 30;152:48-57
pubmed: 27989945
Int J Mol Sci. 2020 Oct 09;21(20):
pubmed: 33050358
Curr Opin Plant Biol. 2021 Jun;61:102060
pubmed: 34087759
Science. 2013 Oct 4;342(6154):118-23
pubmed: 24092744
Cell Biochem Funct. 2016 Jul;34(5):289-98
pubmed: 27003927
Commun Agric Appl Biol Sci. 2012;77(3):151-61
pubmed: 23878969
Plant Physiol Biochem. 2008 Apr;46(4):469-81
pubmed: 17988883
Nucleic Acids Res. 2020 Feb 28;48(4):1790-1799
pubmed: 31819959
BMC Genomics. 2019 Mar 21;20(1):234
pubmed: 30898112
Plant J. 2016 Nov;88(3):361-374
pubmed: 27341062
Cell Mol Life Sci. 2021 May;78(10):4467-4486
pubmed: 33638653
Plant Cell Rep. 2018 Jan;37(1):17-23
pubmed: 28756583
J Exp Bot. 2019 Jan 1;70(1):255-268
pubmed: 30204899
J Exp Bot. 2020 Aug 17;71(17):5256-5268
pubmed: 32060527
BMC Genomics. 2010 Feb 18;11:117
pubmed: 20167053
Microorganisms. 2020 Feb 08;8(2):
pubmed: 32046339
Int J Mol Sci. 2021 Feb 18;22(4):
pubmed: 33670556
Front Microbiol. 2021 Dec 28;12:750089
pubmed: 35027912
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Trends Plant Sci. 2018 Sep;23(9):833-844
pubmed: 29970339
Genome Biol. 2017 Jun 27;18(1):124
pubmed: 28655328
Planta. 2019 Apr;249(4):1087-1105
pubmed: 30547240
Cell Microbiol. 2012 Jun;14(6):829-39
pubmed: 22405188
Nucleic Acids Res. 2023 May 22;51(9):4252-4265
pubmed: 36840717
Int J Mol Sci. 2020 Oct 20;21(20):
pubmed: 33092207
Genome Biol. 2018 Oct 31;19(1):181
pubmed: 30382931
Phytopathology. 2007 Jul;97(7):780-6
pubmed: 18943926
Bioinformatics. 2015 Oct 15;31(20):3380-2
pubmed: 26099264
PLoS Genet. 2018 Nov 30;14(11):e1007797
pubmed: 30500810
Nat Plants. 2016 Sep 19;2:16151
pubmed: 27643635
Trends Plant Sci. 2019 Dec;24(12):1094-1101
pubmed: 31699522
Front Plant Sci. 2021 Mar 04;12:631810
pubmed: 33763093
Physiol Plant. 2022 Sep;174(5):e13771
pubmed: 36053855
Sci Rep. 2018 Jan 15;8(1):757
pubmed: 29335535
Plant J. 2008 Feb;53(3):475-87
pubmed: 17988222
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Plants (Basel). 2020 Nov 05;9(11):
pubmed: 33167573
Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22716-21
pubmed: 21149701
New Phytol. 2019 Jan;221(2):1023-1035
pubmed: 30256420
Environ Microbiol Rep. 2021 Aug;13(4):445-457
pubmed: 33876568
Environ Mol Mutagen. 2008 Jan;49(1):61-72
pubmed: 17948278
Sci Rep. 2018 Sep 28;8(1):14538
pubmed: 30266912
Funct Integr Genomics. 2012 Jun;12(2):379-86
pubmed: 22246600
J Exp Bot. 2008;59(12):3371-81
pubmed: 18648103
Front Plant Sci. 2019 Dec 18;10:1587
pubmed: 31956325
Front Plant Sci. 2016 Mar 30;7:382
pubmed: 27066032
Mol Plant Microbe Interact. 2021 Sep;34(9):990-1000
pubmed: 34010013
FEBS Lett. 2004 Aug 27;573(1-3):83-92
pubmed: 15327980
J Integr Plant Biol. 2021 Mar;63(3):438-450
pubmed: 33421288
Gene. 2022 Aug 30;837:146693
pubmed: 35738444