Contrasting regulatory effects of organic acids on aerobic vinyl chloride biodegradation in etheneotrophs.
Bioremediation
Diauxic growth
Etheneotroph
Global regulatory mechanisms
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
12
05
2022
accepted:
24
08
2022
revised:
17
08
2022
pubmed:
3
9
2022
medline:
15
9
2022
entrez:
2
9
2022
Statut:
ppublish
Résumé
Vinyl chloride (VC) is a common groundwater pollutant generated during anaerobic biodegradation of chlorinated solvents (e.g., trichloroethene (TCE) or tetrachloroethene (PCE)). Aerobic VC biodegradation by etheneotrophs can support anaerobic PCE and TCE bioremediation to achieve complete removal in situ. However, anaerobic bioremediation strategies necessitate biostimulation with electron donors that are fermented in situ, generating organic acids that could influence aerobic VC biodegradation processes. We examined the effect of organic acids (lactate, acetate, propionate, and butyrate) on aerobic VC biodegradation by VC-assimilating etheneotrophs Mycobacterium strain JS60 and Nocardioides strain JS614. Strain JS60 grew on all organic acids tested, while strain JS614 did not respond to lactate. VC-grown strain JS60 fed VC and one or more organic acids showed carbon catabolite repression (CCR) behavior where VC biodegradation occurred only after organic acids were depleted. In contrast, CCR was not evident in VC-grown strain JS614, which degraded VC and organic acids simultaneously. Acetate-grown JS60 showed similar CCR behavior when fed VC and a single organic acid, except that extended lag periods (5-12 days) occurred before VC oxidation ensued. Acetate-grown JS614 fed VC and either acetate or butyrate displayed 5-8 day lag periods before simultaneous VC and organic acid biodegradation. In contrast, acetate-grown JS614 degraded VC and propionate without a significant lag, suggesting a regulatory link between propionate and VC oxidation in JS614. Different global regulatory mechanisms controlling VC biodegradation in the presence of organic acids in etheneotrophs have implications for developing combined anaerobic-aerobic bioremediation strategies at chlorinated ethene-contaminated sites. KEY POINTS: • With organic acids present, VC utilization was repressed in JS60, but not in JS614 • Strain JS60 grew readily on lactate, while strain JS614 did not • Propionate alleviated lag periods for VC utilization in acetate-grown JS614.
Identifiants
pubmed: 36056199
doi: 10.1007/s00253-022-12147-y
pii: 10.1007/s00253-022-12147-y
doi:
Substances chimiques
Butyrates
0
Lactates
0
Propionates
0
Water Pollutants, Chemical
0
Vinyl Chloride
WD06X94M2D
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6335-6346Subventions
Organisme : NIEHS NIH HHS
ID : P30 ES005605
Pays : United States
Organisme : National Science Foundation (US)
ID : 1802583
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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