Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs.

CRISPR-Cas Systems / genetics Gene Library Genes, Lethal RNA, Guide, Kinetoplastida / genetics Synthetic Lethal Mutations
Biotechnology and bioengineering CRISPR High Throughput Screening Molecular Biology

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
16 09 2022
Historique:
entrez: 5 9 2022
pubmed: 6 9 2022
medline: 9 9 2022
Statut: epublish

Résumé

Combinatorial CRISPR screening is useful for investigating synthetic lethality (SL) gene pairs. Here, we detail the steps for dual-gRNA library construction, with the introduction of two backbones, LentiGuide_DKO and LentiCRISPR_DKO. We describe steps for

Identifiants

DOI: 10.1016/j.xpro.2022.101556 PMID: 36060092 PMC: PMC9428847
pubmed: 36060092
doi: 10.1016/j.xpro.2022.101556
pii: S2666-1667(22)00436-1
pmc: PMC9428847
pii:
doi:

Substances chimiques

RNA, Guide 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

101556

Subventions

Organisme : NCI NIH HHS
ID : R01 CA157429
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA196634
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA264652
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA256893
Pays : United States

Informations de copyright

© 2022 The Authors.

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

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Auteurs

Shan Tang (S)

College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA. Electronic address: shan.tang@osumc.edu.

Xue Wu (X)

Department of Biomedical Informatics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.

Jinghui Liu (J)

Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40536, USA.

Qiongsi Zhang (Q)

Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40536, USA.

Xinyi Wang (X)

Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40536, USA.

Shuai Shao (S)

College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.

Birkan Gokbag (B)

Department of Biomedical Informatics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.

Kunjie Fan (K)

Department of Biomedical Informatics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.

Xiaoqi Liu (X)

Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40536, USA.

Fuhai Li (F)

Institute for Informatics and Department of Pediatrics, Washington University in St. Louis, St. Louis, MO 63110, USA.

Lijun Cheng (L)

Department of Biomedical Informatics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.

Lang Li (L)

Department of Biomedical Informatics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA. Electronic address: lang.li@osumc.edu.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Generation of dual-gRNA library for...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Banque de gènes › Generation of dual-gRNA library for...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Gènes létaux › Generation of dual-gRNA library for...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › ARN guide › Generation of dual-gRNA library for...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Mutations synthétiques létales › Generation of dual-gRNA library for...