PhRHMs play important roles in leaf and flower development and anthocyanin synthesis in petunia.
Petunia
/ genetics
Anthocyanins
/ metabolism
Rhamnose
/ metabolism
Arabinose
/ metabolism
Green Fluorescent Proteins
/ genetics
Gene Expression Regulation, Plant
Flowers
/ genetics
Plant Leaves
/ metabolism
Flavonoids
/ metabolism
Oxidoreductases
/ metabolism
Glucose
/ metabolism
Nucleotides
/ metabolism
Racemases and Epimerases
/ genetics
Journal
Physiologia plantarum
ISSN: 1399-3054
Titre abrégé: Physiol Plant
Pays: Denmark
ID NLM: 1256322
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
revised:
24
08
2022
received:
03
07
2022
accepted:
25
08
2022
pubmed:
7
9
2022
medline:
27
10
2022
entrez:
6
9
2022
Statut:
ppublish
Résumé
Anthocyanins, vital metabolites in plants, are formed by anthocyanidins combined with various monosaccharides, including glucose, rhamnose, and arabinose. Rhamnose contributes greatly to the glycosylation of anthocyanidins. There are two kinds of rhamnose synthase (RS): rhamnose biosynthesis (RHM), and nucleotide-RS/epimerase-reductase (UER1). Nevertheless, no RS isoform was reported to be involved in anthocyanin synthesis. Here, three homologous PhRHM genes, namely PhRHM1, PhRHM2, and PhRHM3, and one PhUER1 gene from petunia were cloned and characterized. Green fluorescent protein fusion protein assays revealed that PhRHMs and PhUER1 are localized in the cytoplasm. We obtained PhRHM1 or/and PhRHM2 or PhUER1 silenced petunia plants and did not attempt to obtain PhRHM3 silenced plants since PhRHM3 mRNA was not detected in petunia organs examined. PhRHM1 and PhRHM2 (PhRHM1-2) silencing induced abnormal plant growth and decreased the contents of l-rhamnose, photosynthetic pigments and total anthocyanins, while PhUER1 silencing did not cause any visible phenotypic changes. Flavonoid metabolome analysis further revealed that PhRHM1-2 silencing reduced the contents of anthocyanins with rhamnose residue. These results revealed that PhRHMs contribute to the biosynthesis of rhamnose and that PhRHMs participate in the anthocyanin rhamnosylation in petunia, while PhUER1 does not.
Substances chimiques
Anthocyanins
0
Rhamnose
QN34XC755A
Arabinose
B40ROO395Z
Green Fluorescent Proteins
147336-22-9
Flavonoids
0
Oxidoreductases
EC 1.-
Glucose
IY9XDZ35W2
Nucleotides
0
Racemases and Epimerases
EC 5.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13773Subventions
Organisme : National Key Research and Development Plan
ID : 2018YFD1000407
Organisme : National Natural Science Foundation of China
ID : 31872137
Organisme : National Natural Science Foundation of China
ID : 31770737
Organisme : National Natural Science Foundation of China
ID : 31870692
Informations de copyright
© 2022 Scandinavian Plant Physiology Society.
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