The human disease gene LYSET is essential for lysosomal enzyme transport and viral infection.


Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
07 10 2022
Historique:
pubmed: 9 9 2022
medline: 12 10 2022
entrez: 8 9 2022
Statut: ppublish

Résumé

Lysosomes are key degradative compartments of the cell. Transport to lysosomes relies on GlcNAc-1-phosphotransferase-mediated tagging of soluble enzymes with mannose 6-phosphate (M6P). GlcNAc-1-phosphotransferase deficiency leads to the severe lysosomal storage disorder mucolipidosis II (MLII). Several viruses require lysosomal cathepsins to cleave structural proteins and thus depend on functional GlcNAc-1-phosphotransferase. We used genome-scale CRISPR screens to identify lysosomal enzyme trafficking factor (LYSET, also named TMEM251) as essential for infection by cathepsin-dependent viruses including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). LYSET deficiency resulted in global loss of M6P tagging and mislocalization of GlcNAc-1-phosphotransferase from the Golgi complex to lysosomes.

Identifiants

pubmed: 36074821
doi: 10.1126/science.abn5648
pmc: PMC9547973
mid: NIHMS1836118
doi:

Substances chimiques

LYSET protein, human 0
Proteins 0
Transferases (Other Substituted Phosphate Groups) EC 2.7.8.-
Cathepsins EC 3.4.-
Mannose PHA4727WTP

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eabn5648

Subventions

Organisme : NCI NIH HHS
ID : K00 CA212454
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI141970
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM058867
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007276
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI130123
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI140186
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007502
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI134907
Pays : United States

Références

Hum Mutat. 2021 Jan;42(1):89-101
pubmed: 33252156
Nature. 2022 Mar;603(7902):706-714
pubmed: 35104837
Nat Protoc. 2007;2(10):2480-91
pubmed: 17947990
Nat Med. 2005 Oct;11(10):1109-12
pubmed: 16200072
Hum Mutat. 2020 Jul;41(7):1321-1328
pubmed: 32220096
Anal Chem. 2014 Jun 3;86(11):5478-86
pubmed: 24796651
Biochim Biophys Acta. 2009 Apr;1793(4):605-14
pubmed: 19046998
Proteomes. 2021 Jan 26;9(1):
pubmed: 33530589
Development. 2001 Dec;128(23):4899-910
pubmed: 11731469
J Proteome Res. 2018 Sep 7;17(9):2917-2924
pubmed: 30114372
Traffic. 2015 Jul;16(7):743-59
pubmed: 25786328
Cell Host Microbe. 2020 Sep 9;28(3):486-496.e6
pubmed: 32738193
J Proteome Res. 2020 Aug 7;19(8):3286-3301
pubmed: 32500713
Nat Microbiol. 2019 Dec;4(12):2523-2537
pubmed: 31527793
Nature. 2011 Aug 24;477(7364):340-3
pubmed: 21866103
Science. 2011 Jul 1;333(6038):87-90
pubmed: 21719679
Annu Rev Biochem. 2010;79:803-33
pubmed: 20196649
Anal Chem. 2019 Aug 20;91(16):10401-10406
pubmed: 31287300
Methods Enzymol. 2018;598:217-235
pubmed: 29306436
Cell. 2021 Jan 7;184(1):106-119.e14
pubmed: 33333024
Int J Mol Sci. 2020 Sep 17;21(18):
pubmed: 32957425
Curr Protoc Bioinformatics. 2012 Dec;Chapter 13:Unit13.20
pubmed: 23255153
Nat Commun. 2020 Nov 18;11(1):5885
pubmed: 33208793
Nat Biotechnol. 2016 Feb;34(2):184-191
pubmed: 26780180
Nature. 2020 May;581(7809):434-443
pubmed: 32461654
J Am Chem Soc. 2013 Oct 2;135(39):14726-30
pubmed: 23971698
Mol Cell Proteomics. 2019 Jan;18(1):16-27
pubmed: 30237200
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14764-9
pubmed: 9405687
Nature. 2003 Nov 27;426(6965):450-4
pubmed: 14647384
Sci Data. 2020 Feb 26;7(1):68
pubmed: 32103020
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
DNA Cell Biol. 2012 Dec;31(12):1646-9
pubmed: 23134451
Genome Res. 2014 Dec;24(12):2059-65
pubmed: 25373145
J Virol. 2010 Jan;84(1):163-75
pubmed: 19846533
Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11876-81
pubmed: 16081529
Nucleic Acids Res. 2019 Jan 8;47(D1):D506-D515
pubmed: 30395287
Mol Cell Proteomics. 2017 Jul;16(7):1173-1187
pubmed: 28539326
Biol Chem. 2002 May;383(5):751-6
pubmed: 12108539
J Clin Invest. 2000 Mar;105(5):673-81
pubmed: 10712439
Science. 1967 Aug 18;157(3790):804-6
pubmed: 17842782
Genome Biol. 2014;15(12):554
pubmed: 25476604
Nat Biotechnol. 2016 Nov;34(11):1198-1205
pubmed: 27748755
PLoS One. 2009 Aug 06;4(8):e6529
pubmed: 19657394
J Biol Chem. 2013 Jan 11;288(2):1238-49
pubmed: 23192343
Science. 2005 Jun 10;308(5728):1643-5
pubmed: 15831716
J Proteome Res. 2012 Sep 7;11(9):4517-25
pubmed: 22800195
Cell Host Microbe. 2020 May 13;27(5):841-848.e3
pubmed: 32289263
Invest Ophthalmol Vis Sci. 2007 Nov;48(11):5221-8
pubmed: 17962477
Eur J Cell Biol. 1992 Apr;57(2):184-92
pubmed: 1387363
Nat Methods. 2014 Aug;11(8):783-784
pubmed: 25075903
Hum Mutat. 2014 Mar;35(3):368-76
pubmed: 24375680
Glycobiology. 2001 Aug;11(8):633-44
pubmed: 11479274
J Virol. 2013 Dec;87(23):12552-61
pubmed: 24027332
Bioinformatics. 2010 Apr 1;26(7):966-8
pubmed: 20147306
Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):3532-7
pubmed: 24550498
Curr Protoc Cell Biol. 2015 Dec 01;69:3.1.1-3.1.22
pubmed: 26621372
Mass Spectrom Rev. 2020 May;39(3):229-244
pubmed: 28691345
J Biol Chem. 2002 Jul 5;277(27):24609-17
pubmed: 11986312
Anal Biochem. 1986 May 1;154(2):655-63
pubmed: 3728974
J Microsc. 2006 Dec;224(Pt 3):213-32
pubmed: 17210054
Nucleic Acids Res. 2019 Jan 8;47(D1):D419-D426
pubmed: 30407594
Nature. 2022 Mar;603(7902):715-720
pubmed: 35104836
Nat Protoc. 2013 Nov;8(11):2281-2308
pubmed: 24157548
Am J Pathol. 2010 Jul;177(1):240-7
pubmed: 20472886
Mol Biol Cell. 2011 Apr 15;22(8):1135-47
pubmed: 21325625
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
Int J Proteomics. 2012;2012:560391
pubmed: 22701174

Auteurs

Christopher M Richards (CM)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Sabrina Jabs (S)

Institute of Clinical Molecular Biology, Christian-Albrechts-University and University Medical Center Schleswig-Holstein, Campus Kiel, Kiel, Germany.

Wenjie Qiao (W)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Lauren D Varanese (LD)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Michaela Schweizer (M)

Department of Electron Microscopy, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Peter R Mosen (PR)

Institute for Biochemistry and Molecular Biology, Medical Faculty, Rheinische Friedrich-Wilhelms-University of Bonn, Bonn, Germany.

Nicholas M Riley (NM)

Department of Chemistry, Stanford University, Stanford, CA, USA.

Malte Klüssendorf (M)

Department of Osteology and Biomechanics, Cell Biology of Rare Diseases, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

James R Zengel (JR)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Ryan A Flynn (RA)

Stem Cell Program, Boston Children's Hospital, Boston, MA, USA.
Stem Cell and Regenerative Biology Department, Harvard University, Cambridge, MA, USA.

Arjun Rustagi (A)

Division of Infectious Disease and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.

John C Widen (JC)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Christine E Peters (CE)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Yaw Shin Ooi (YS)

Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore.

Xuping Xie (X)

Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.

Pei-Yong Shi (PY)

Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.

Ralf Bartenschlager (R)

Department of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, Germany.
Division Virus-Associated Carcinogenesis, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Andreas S Puschnik (AS)

Chan Zuckerberg Biohub, San Francisco, CA, USA.

Matthew Bogyo (M)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.
Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Carolyn R Bertozzi (CR)

Department of Chemistry, Stanford University, Stanford, CA, USA.
Howard Hughes Medical Institute, Stanford, CA, USA.

Catherine A Blish (CA)

Division of Infectious Disease and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.

Dominic Winter (D)

Institute for Biochemistry and Molecular Biology, Medical Faculty, Rheinische Friedrich-Wilhelms-University of Bonn, Bonn, Germany.

Claude M Nagamine (CM)

Department of Comparative Medicine, Stanford University School of Medicine, Stanford, CA, USA.

Thomas Braulke (T)

Department of Osteology and Biomechanics, Cell Biology of Rare Diseases, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Jan E Carette (JE)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH