Initiating DNA-Encoded Library Synthesis with a Hexathymidine DNA Oligonucleotide.
Adapter oligonucleotide
Au(I)-mediated heterocycle synthesis
DNA ligation
DNA-encoded chemistry
DNA-encoded library
Hexathymidine
Pictet-Spengler reaction
Solid-phase synthesis
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
9
9
2022
pubmed:
10
9
2022
medline:
14
9
2022
Statut:
ppublish
Résumé
Libraries of DNA-encoded compounds (DELs) are a validated screening technology for drug discovery. Here we describe a library synthesis strategy that starts with a solid phase-bound, chemically very stable hexathymidine DNA sequence "hexT." Different heterocycle conjugates of the hexT oligonucleotide were synthesized from simple starting materials using metal or acid catalysts. The hexT conjugates were isolated, characterized, and ligated to coding DNA sequences.
Identifiants
pubmed: 36083549
doi: 10.1007/978-1-0716-2545-3_14
doi:
Substances chimiques
Oligonucleotides
0
Small Molecule Libraries
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
89-104Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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