Erythrocytes from patients with ST-elevation myocardial infarction induce cardioprotection through the purinergic P2Y
Adenosine Triphosphate
Animals
Cyclic GMP-Dependent Protein Kinases
Erythrocytes
/ metabolism
Humans
Myocardial Infarction
/ prevention & control
Myocardial Reperfusion Injury
/ prevention & control
NG-Nitroarginine Methyl Ester
/ pharmacology
Nitric Oxide
/ metabolism
Nitric Oxide Synthase
Purinergic P2 Receptor Antagonists
Rats
Receptors, Purinergic P2
/ metabolism
ST Elevation Myocardial Infarction
/ metabolism
Soluble Guanylyl Cyclase
ATP
Ischemia–reperfusion injury
Nitric oxide
Purinergic
Red blood cells
ST-elevation myocardial infarction
Soluble guanylyl cyclase
Journal
Basic research in cardiology
ISSN: 1435-1803
Titre abrégé: Basic Res Cardiol
Pays: Germany
ID NLM: 0360342
Informations de publication
Date de publication:
16 09 2022
16 09 2022
Historique:
received:
22
09
2021
accepted:
06
09
2022
revised:
05
09
2022
entrez:
16
9
2022
pubmed:
17
9
2022
medline:
21
9
2022
Statut:
epublish
Résumé
Red blood cells (RBCs) are suggested to play a role in cardiovascular regulation by exporting nitric oxide (NO) bioactivity and ATP under hypoxia. It remains unknown whether such beneficial effects of RBCs are protective in patients with acute myocardial infarction. We investigated whether RBCs from patients with ST-elevation myocardial infarction (STEMI) protect against myocardial ischemia-reperfusion injury and whether such effect involves NO and purinergic signaling in the RBCs. RBCs from patients with STEMI undergoing primary coronary intervention and healthy controls were administered to isolated rat hearts subjected to global ischemia and reperfusion. Compared to RBCs from healthy controls, RBCs from STEMI patients reduced myocardial infarct size (30 ± 12% RBC healthy vs. 11 ± 5% RBC STEMI patients, P < 0.001), improved recovery of left-ventricular developed pressure and dP/dt and reduced left-ventricular end-diastolic pressure in hearts subjected to ischemia-reperfusion. Inhibition of RBC NO synthase with L-NAME or soluble guanylyl cyclase (sGC) with ODQ, and inhibition of cardiac protein kinase G (PKG) abolished the cardioprotective effect. Furthermore, the non-selective purinergic P2 receptor antagonist PPADS but not the P1 receptor antagonist 8PT attenuated the cardioprotection induced by RBCs from STEMI patients. The P2Y
Identifiants
pubmed: 36112326
doi: 10.1007/s00395-022-00953-4
pii: 10.1007/s00395-022-00953-4
pmc: PMC9481504
doi:
Substances chimiques
P2RY13 protein, human
0
Purinergic P2 Receptor Antagonists
0
Receptors, Purinergic P2
0
Nitric Oxide
31C4KY9ESH
Adenosine Triphosphate
8L70Q75FXE
Nitric Oxide Synthase
EC 1.14.13.39
Cyclic GMP-Dependent Protein Kinases
EC 2.7.11.12
Soluble Guanylyl Cyclase
EC 4.6.1.2
NG-Nitroarginine Methyl Ester
V55S2QJN2X
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
46Informations de copyright
© 2022. The Author(s).
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