Vasoconstrictor and hemodynamic effects of a methanolic extract from Rhinella marina toad poison.
Adenosine Triphosphatases
/ metabolism
Adrenergic Agents
/ pharmacology
Adrenergic Antagonists
/ pharmacology
Animals
Bufanolides
/ toxicity
Bufo marinus
/ metabolism
Calcium Channels
Endothelium, Vascular
Hemodynamics
Indomethacin
/ pharmacology
Ketanserin
/ pharmacology
Male
Methanol
/ pharmacology
NADPH Oxidases
NG-Nitroarginine Methyl Ester
Nifedipine
/ pharmacology
Nitric Oxide
/ metabolism
Poisons
Prazosin
/ pharmacology
Prostaglandin-Endoperoxide Synthases
/ metabolism
Rats
Rats, Wistar
Serotonin
/ pharmacology
Vasoconstrictor Agents
Amphibian poisons
Aorta
Rhinella marina
Vascular reactivity
blood pressure
Journal
Toxicon : official journal of the International Society on Toxinology
ISSN: 1879-3150
Titre abrégé: Toxicon
Pays: England
ID NLM: 1307333
Informations de publication
Date de publication:
30 Oct 2022
30 Oct 2022
Historique:
received:
04
05
2022
revised:
10
08
2022
accepted:
28
08
2022
pubmed:
17
9
2022
medline:
28
9
2022
entrez:
16
9
2022
Statut:
ppublish
Résumé
Rhinella marina toad is abundant in Brazil. Its poison contains cardiac glycosides called bufadienolides, which are extensively investigated for their bioactivity. Our aim was to characterize the vasoactivity of Rhinella marina poison (RmP) on the aorta of male Wistar rats. For this, the RmP was first collected and processed to obtain an alcoholic extract. To determine cardiovascular effects of RmP, we performed in vivo tests by administering RmP intravenously in doses of 0.1-0.8 mg/kg. Vascular reactivity was also performed through concentration-response curves to RmP (10 ng/mL to 200 μg/mL) in aortic segments with and without endothelium. RmP induced a concentration-dependent contraction in rat aorta which was partly endothelium-mediated. Nitric oxide contributes with this response in view that incubation with L-NAME increased the contractile response. Additionally, treatment with indomethacin [cyclooxygenase, (COX) inhibitor], nifedipine (L-type voltage-gated calcium channels blocker), and BQ-123 (ET
Identifiants
pubmed: 36113683
pii: S0041-0101(22)00252-5
doi: 10.1016/j.toxicon.2022.08.018
pmc: PMC9832923
mid: NIHMS1850629
pii:
doi:
Substances chimiques
Adrenergic Agents
0
Adrenergic Antagonists
0
Bufanolides
0
Calcium Channels
0
Poisons
0
Vasoconstrictor Agents
0
Nitric Oxide
31C4KY9ESH
Serotonin
333DO1RDJY
Ketanserin
97F9DE4CT4
Prostaglandin-Endoperoxide Synthases
EC 1.14.99.1
NADPH Oxidases
EC 1.6.3.-
Adenosine Triphosphatases
EC 3.6.1.-
Nifedipine
I9ZF7L6G2L
NG-Nitroarginine Methyl Ester
V55S2QJN2X
Prazosin
XM03YJ541D
Indomethacin
XXE1CET956
Methanol
Y4S76JWI15
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
57-65Subventions
Organisme : NIGMS NIH HHS
ID : R00 GM118885
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL149762
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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