Cryopreservation of Whole Rat Livers by Vitrification and Nanowarming.
Critical cooling rate
Critical warming rate
Cryoprotective agent
Liver transplant
Organ preservation
Journal
Annals of biomedical engineering
ISSN: 1573-9686
Titre abrégé: Ann Biomed Eng
Pays: United States
ID NLM: 0361512
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
11
04
2022
accepted:
22
08
2022
pmc-release:
01
03
2024
pubmed:
2
10
2022
medline:
17
2
2023
entrez:
1
10
2022
Statut:
ppublish
Résumé
Liver cryopreservation has the potential to enable indefinite organ banking. This study investigated vitrification-the ice-free cryopreservation of livers in a glass-like state-as a promising alternative to conventional cryopreservation, which uniformly fails due to damage from ice formation or cracking. Our unique "nanowarming" technology, which involves perfusing biospecimens with cryoprotective agents (CPAs) and silica-coated iron oxide nanoparticles (sIONPs) and then, after vitrification, exciting the nanoparticles via radiofrequency waves, enables rewarming of vitrified specimens fast enough to avoid ice formation and uniformly enough to prevent cracking from thermal stresses, thereby addressing the two main failures of conventional cryopreservation. This study demonstrates the ability to load rat livers with both CPA and sIONPs by vascular perfusion, cool them rapidly to an ice-free vitrified state, and rapidly and homogenously rewarm them. While there was some elevation of liver enzymes (Alanine Aminotransferase) and impaired indocyanine green (ICG) excretion, the nanowarmed livers were viable, maintained normal tissue architecture, had preserved vascular endothelium, and demonstrated hepatocyte and organ-level function, including production of bile and hepatocyte uptake of ICG during normothermic reperfusion. These findings suggest that cryopreservation of whole livers via vitrification and nanowarming has the potential to achieve organ banking for transplant and other biomedical applications.
Identifiants
pubmed: 36183025
doi: 10.1007/s10439-022-03064-2
pii: 10.1007/s10439-022-03064-2
pmc: PMC10315167
mid: NIHMS1841057
doi:
Substances chimiques
Cryoprotective Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
566-577Subventions
Organisme : NHLBI NIH HHS
ID : HL135046
Pays : United States
Organisme : NIDDK NIH HHS
ID : DK117425
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK117425
Pays : United States
Organisme : NIDDK NIH HHS
ID : R43 DK126551
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK131209
Pays : United States
Organisme : NIDDK NIH HHS
ID : DK126551
Pays : United States
Organisme : NIDDK NIH HHS
ID : DK131209
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL135046
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2022. The Author(s) under exclusive licence to Biomedical Engineering Society.
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