Bromodomain Interactions with Acetylated Histone 4 Peptides in the BRD4 Tandem Domain: Effects on Domain Dynamics and Internal Flexibility.
Journal
Biochemistry
ISSN: 1520-4995
Titre abrégé: Biochemistry
Pays: United States
ID NLM: 0370623
Informations de publication
Date de publication:
01 11 2022
01 11 2022
Historique:
pubmed:
11
10
2022
medline:
3
11
2022
entrez:
10
10
2022
Statut:
ppublish
Résumé
The bromodomain and extra-terminal (BET) protein BRD4 regulates gene expression via recruitment of transcriptional regulatory complexes to acetylated chromatin. Like other BET proteins, BRD4 contains two bromodomains, BD1 and BD2, that can interact cooperatively with target proteins and designed ligands, with important implications for drug discovery. Here, we used nuclear magnetic resonance (NMR) spectroscopy to study the dynamics and interactions of the isolated bromodomains, as well as the tandem construct including both domains and the intervening linker, and investigated the effects of binding a tetra-acetylated peptide corresponding to the tail of histone 4. The peptide affinity is lower for both domains in the tandem construct than for the isolated domains. Using
Identifiants
pubmed: 36215732
doi: 10.1021/acs.biochem.2c00226
pmc: PMC9631989
doi:
Substances chimiques
Histones
0
Nuclear Proteins
0
Transcription Factors
0
Peptides
0
Cell Cycle Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2303-2318Références
Angew Chem Int Ed Engl. 2013 Apr 2;52(14):3911-5
pubmed: 23450638
J Biomol NMR. 2002 Jun;23(2):139-50
pubmed: 12153039
Science. 2020 Apr 24;368(6489):387-394
pubmed: 32193360
J Biol Chem. 2010 Mar 5;285(10):7610-8
pubmed: 20048151
J Biomol NMR. 1999 Oct;15(2):181-4
pubmed: 10605091
Nature. 2010 Dec 23;468(7327):1067-73
pubmed: 20871596
Nature. 2010 Dec 23;468(7327):1119-23
pubmed: 21068722
Nat Struct Mol Biol. 2007 Nov;14(11):1025-1040
pubmed: 17984965
Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19754-9
pubmed: 24248379
Proteins. 2005 Jun 1;59(4):687-96
pubmed: 15815974
Biophys J. 2010 Oct 20;99(8):2636-46
pubmed: 20959105
J Magn Reson. 2008 Feb;190(2):333-8
pubmed: 18078771
Proc Natl Acad Sci U S A. 2018 Jul 31;115(31):7949-7954
pubmed: 30012592
J Magn Reson. 2001 Apr;149(2):204-17
pubmed: 11318619
Nat Chem Biol. 2016 Dec;12(12):1089-1096
pubmed: 27775715
J Magn Reson. 2004 Jun;168(2):336-45
pubmed: 15140445
Commun Biol. 2021 Nov 9;4(1):1273
pubmed: 34754068
Nat Struct Mol Biol. 2019 Oct;26(10):870-879
pubmed: 31582847
J Biomol NMR. 2012 Apr;52(4):315-27
pubmed: 22331404
Science. 2014 Oct 31;346(6209):638-641
pubmed: 25323695
Mol Biosyst. 2007 Jul;3(7):483-94
pubmed: 17579774
J Biol Chem. 2012 Mar 30;287(14):10738-52
pubmed: 22334664
J Am Chem Soc. 2010 Mar 31;132(12):4295-309
pubmed: 20199098
J Phys Chem B. 2014 Sep 25;118(38):11120-8
pubmed: 25167331
J Biomol NMR. 1995 Sep;6(2):153-62
pubmed: 8589604
J Chem Phys. 2009 Dec 14;131(22):224507
pubmed: 20001057
J Magn Reson. 2000 Apr;143(2):423-6
pubmed: 10729271
J Mol Biol. 2000 Jan 28;295(4):963-78
pubmed: 10656804
Proteins. 2020 Mar;88(3):414-430
pubmed: 31587361
J Biomol NMR. 2015 Feb;61(2):109-21
pubmed: 25577242
Magn Reson Chem. 2007 Feb;45(2):175-8
pubmed: 17154329
J Biomol NMR. 2008 Feb;40(2):107-19
pubmed: 18085410
J Biomol NMR. 1995 Nov;6(3):277-93
pubmed: 8520220
J Mol Biol. 1998 Jul 31;280(5):933-52
pubmed: 9671561
PLoS One. 2016 Apr 29;11(4):e0154607
pubmed: 27128490
J Biomol NMR. 2013 Apr;55(4):355-67
pubmed: 23456039
Nature. 1999 Jun 3;399(6735):491-6
pubmed: 10365964
J Am Chem Soc. 2005 Jun 8;127(22):8014-5
pubmed: 15926816
J Biomol NMR. 1991 May;1(1):99-104
pubmed: 1668719
Proc Natl Acad Sci U S A. 2020 Oct 27;117(43):26728-26738
pubmed: 33046654
Biochemistry. 1989 Nov 14;28(23):8972-9
pubmed: 2690953
J Med Chem. 2016 Feb 25;59(4):1634-41
pubmed: 26735842
Methods Enzymol. 2001;339:204-38
pubmed: 11462813
J Biol Chem. 2007 May 4;282(18):13141-5
pubmed: 17329240
Phys Chem Chem Phys. 2017 Sep 13;19(35):23934-23941
pubmed: 28849824
J Biol Chem. 2009 Dec 25;284(52):36547-36556
pubmed: 19828451
J Biol Chem. 2007 Feb 9;282(6):4193-201
pubmed: 17148447
Biochemistry. 2021 Mar 9;60(9):648-662
pubmed: 33620209
Biochemistry. 2004 May 25;43(20):5930-6
pubmed: 15147176
PLoS One. 2017 Oct 27;12(10):e0186570
pubmed: 29077715
J Magn Reson. 2000 Nov;147(1):138-46
pubmed: 11042057
FEBS Lett. 2013 Jul 11;587(14):2158-63
pubmed: 23711371
J Magn Reson. 2006 Jan;178(1):72-6
pubmed: 16188473
Biochemistry. 2008 Jun 17;47(24):6403-17
pubmed: 18500820
Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12135-40
pubmed: 14528004
Mol Cell. 2014 Jun 5;54(5):728-36
pubmed: 24905006
Nat Chem Biol. 2016 Dec;12(12):1097-1104
pubmed: 27775716
Cell. 2012 Mar 30;149(1):214-31
pubmed: 22464331
PLoS One. 2016 Jul 12;11(7):e0159180
pubmed: 27403962
J Biol Chem. 2012 Jan 6;287(2):1090-9
pubmed: 22084242
Nature. 2009 Oct 1;461(7264):664-8
pubmed: 19794495
J Biol Chem. 2012 Aug 17;287(34):28840-51
pubmed: 22645123
J Magn Reson. 2011 Dec;213(2):423-41
pubmed: 22152361
Proc Natl Acad Sci U S A. 2021 Jan 26;118(4):
pubmed: 33468647