Identification and biochemical characterization of a novel N-acetylglucosamine kinase in Saccharomyces cerevisiae.
Acetylglucosamine
/ metabolism
Adenosine Triphosphate
/ metabolism
Cytidine Triphosphate
/ metabolism
Glucose
/ metabolism
Glycoproteins
/ metabolism
Guanosine Triphosphate
/ metabolism
Nucleosides
/ metabolism
Phosphates
/ metabolism
Phosphotransferases (Alcohol Group Acceptor)
/ genetics
Polysaccharides
/ metabolism
Saccharomyces cerevisiae
/ enzymology
Uridine Triphosphate
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
10 10 2022
10 10 2022
Historique:
received:
09
06
2022
accepted:
27
09
2022
entrez:
10
10
2022
pubmed:
11
10
2022
medline:
13
10
2022
Statut:
epublish
Résumé
N-acetylglucosamine (GlcNAc) is a key component of glycans such as glycoprotein and the cell wall. GlcNAc kinase is an enzyme that transfers a phosphate onto GlcNAc to generate GlcNAc-6-phosphate, which can be a precursor for glycan synthesis. GlcNAc kinases have been found in a broad range of organisms, including pathogenic yeast, human and bacteria. However, this enzyme has never been discovered in Saccharomyces cerevisiae, a eukaryotic model. In this study, the first GlcNAc kinase from S. cerevisiae was identified and named Ngk1. The K
Identifiants
pubmed: 36216916
doi: 10.1038/s41598-022-21400-3
pii: 10.1038/s41598-022-21400-3
pmc: PMC9550789
doi:
Substances chimiques
Glycoproteins
0
Nucleosides
0
Phosphates
0
Polysaccharides
0
Cytidine Triphosphate
65-47-4
Guanosine Triphosphate
86-01-1
Adenosine Triphosphate
8L70Q75FXE
Phosphotransferases (Alcohol Group Acceptor)
EC 2.7.1.-
N-acetylglucosamine kinase
EC 2.7.1.59
Glucose
IY9XDZ35W2
Uridine Triphosphate
UT0S826Z60
Acetylglucosamine
V956696549
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16991Informations de copyright
© 2022. The Author(s).
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