A
Authors and affiliations.
Bone Morphogenetic Proteins
Humans
Inflammation Mediators
/ metabolism
Macrophage Colony-Stimulating Factor
Macrophages
/ metabolism
Matrix Metalloproteinase 8
/ metabolism
Matrix Metalloproteinase 9
Purinergic P1 Receptor Agonists
/ metabolism
Receptors, Purinergic P1
/ metabolism
Severity of Illness Index
Spondylitis, Ankylosing
/ drug therapy
Adenosine A2A receptor
Ankylosing spondylitis
Bone morphogenetic protein
Macrophages
Matrix metalloproteinase
Journal
BMC musculoskeletal disorders
ISSN: 1471-2474
Titre abrégé: BMC Musculoskelet Disord
Pays: England
ID NLM: 100968565
Informations de publication
Date de publication:
12 Oct 2022
12 Oct 2022
Historique:
received:
10
05
2022
accepted:
23
09
2022
entrez:
11
10
2022
pubmed:
12
10
2022
medline:
14
10
2022
Statut:
epublish
Résumé
Ankylosing spondylitis (AS) is an inflammatory autoimmune disease that mostly affects different joints of the body. Macrophages are the predominant cells that mediate disease progression by secreting several pro-inflammatory mediators. Different receptors are involved in macrophages' function including the adenosine receptors (AR). Our main objective in this study was to assess the effect of applying A Monocytes were isolated from the whole blood of 28 individuals (AS patients and healthy controls in a 1:1 ratio). Macrophages were differentiated using macrophage colony-stimulating factor (M-CSF), and flow cytometry was performed to confirm surface markers. CGS-21,680 was used to treat cells that had been differentiated. Using SYBR green real-time PCR, relative gene expression was determined. Activating A Due to the disability of A
Sections du résumé
BACKGROUND
BACKGROUND
Ankylosing spondylitis (AS) is an inflammatory autoimmune disease that mostly affects different joints of the body. Macrophages are the predominant cells that mediate disease progression by secreting several pro-inflammatory mediators. Different receptors are involved in macrophages' function including the adenosine receptors (AR). Our main objective in this study was to assess the effect of applying A
METHODS
METHODS
Monocytes were isolated from the whole blood of 28 individuals (AS patients and healthy controls in a 1:1 ratio). Macrophages were differentiated using macrophage colony-stimulating factor (M-CSF), and flow cytometry was performed to confirm surface markers. CGS-21,680 was used to treat cells that had been differentiated. Using SYBR green real-time PCR, relative gene expression was determined.
RESULTS
RESULTS
Activating A
CONCLUSION
CONCLUSIONS
Due to the disability of A
Identifiants
pubmed: 36221125
doi: 10.1186/s12891-022-05846-0
pii: 10.1186/s12891-022-05846-0
pmc: PMC9555099
doi:
Substances chimiques
Bone Morphogenetic Proteins
0
Inflammation Mediators
0
Purinergic P1 Receptor Agonists
0
Receptors, Purinergic P1
0
Macrophage Colony-Stimulating Factor
81627-83-0
MMP8 protein, human
EC 3.4.24.34
Matrix Metalloproteinase 8
EC 3.4.24.34
Matrix Metalloproteinase 9
EC 3.4.24.35
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
908Subventions
Organisme : Tehran University of Medical Sciences
ID : 99-3-96-51351
Organisme : Tehran University of Medical Sciences
ID : 99-3-96-51351
Informations de copyright
© 2022. The Author(s).
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