Demonstration of bacterium-free very rapid reverse genetics system using mumps virus.
Escherichia coli-free
OriCiro cell-free cloning system
PCR
mumps virus
reverse genetics
Journal
Microbiology and immunology
ISSN: 1348-0421
Titre abrégé: Microbiol Immunol
Pays: Australia
ID NLM: 7703966
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
revised:
20
09
2022
received:
15
07
2022
accepted:
30
09
2022
pubmed:
20
10
2022
medline:
10
1
2023
entrez:
19
10
2022
Statut:
ppublish
Résumé
The reverse genetics system is a very powerful tool for analyzing the molecular mechanisms of viral propagation and pathogenesis. However, full-length genome plasmid construction is highly time-consuming and laborious, and undesired mutations may be introduced by Escherichia coli. This study shows a very rapid E. coli-free method of full-genome construction using the mumps virus as an example. This method was able to reduce dramatically the time for full-genome construction, which was used very efficiently for virus rescue, from several days or more to ~2 days, with a similar accuracy and yield to the conventional method using E. coli/plasmid.
Identifiants
pubmed: 36259144
doi: 10.1111/1348-0421.13032
pmc: PMC10092663
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
44-47Subventions
Organisme : Japan Society for the Promotion of Science
ID : 19K07584
Organisme : Japan Society for the Promotion of Science
ID : 22K07108
Organisme : Japan Agency for Medical Research and Development
ID : JP21fk0108087j0403
Organisme : Japan Agency for Medical Research and Development
ID : JP21fk0108617j0201
Organisme : Japan Agency for Medical Research and Development
ID : JP21fk0108623j0001
Organisme : Japan Agency for Medical Research and Development
ID : JP21wm0325024j0002
Informations de copyright
© 2022 The Authors. Microbiology and Immunology published by The Societies and John Wiley & Sons Australia, Ltd.
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