Reference genes expression stability in Avena sativa L. during compatible and incompatible interactions with Puccinia graminis.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
01 11 2022
01 11 2022
Historique:
received:
25
08
2022
accepted:
21
10
2022
entrez:
2
11
2022
pubmed:
3
11
2022
medline:
4
11
2022
Statut:
epublish
Résumé
A reliable qPCR experiment requires the selection of reference genes with a stable level of expression in a given experimental system. This study attempts to determine the reference genes (RGs) for the A. sativa-P. graminis experimental setup. We evaluated nine candidate reference genes in A. sativa (oat line Pg4 and the cultivar Kasztan) during compatible and incompatible interactions with different pathotypes of Puccinia graminis f. sp. avenae in six time points post-inoculation. The identification of genes with high expression stability was performed by four algorithms (geNorm, NormFinder, BestKeeper and ΔCt method). We found that the most appropriate combination of RGs for RT-qPCR data normalization were HNR (heterogeneous nuclear ribonucleoprotein 27C) + EF1A (elongation factor 1-alpha) + EIF4A (eukaryotic initiation factor 4A-3). The worst candidates for normalization in this dataset were CYP (cyclophilin) and TUA (alpha tubulin). Identified reference genes are suitable candidates for the standardization of gene expression studies in the A. sativa-P. graminis interaction system and potentially other related pathogens. To date, this is the first report of RGs selection in this pathosystem.
Identifiants
pubmed: 36319744
doi: 10.1038/s41598-022-22993-5
pii: 10.1038/s41598-022-22993-5
pmc: PMC9626582
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
18369Informations de copyright
© 2022. The Author(s).
Références
Plant Pathol. 2017 Apr;66(3):450-459
pubmed: 28356604
PLoS One. 2018 Feb 1;13(2):e0192343
pubmed: 29390041
Plant Methods. 2020 Oct 15;16:138
pubmed: 33072174
Plant Dis. 2019 Jul;103(7):1559-1564
pubmed: 31025903
Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20260-4
pubmed: 22106295
Genes (Basel). 2020 Aug 31;11(9):
pubmed: 32878033
BMC Bioinformatics. 2012 Jun 18;13:134
pubmed: 22708584
Biotechnol Lett. 2004 Mar;26(6):509-15
pubmed: 15127793
Science. 2010 Feb 12;327(5967):812-8
pubmed: 20110467
PLoS One. 2013 Sep 04;8(9):e72998
pubmed: 24023800
Plant Dis. 2007 Jun;91(6):763-766
pubmed: 30780488
PLoS One. 2014 Dec 11;9(12):e115206
pubmed: 25503716
BMC Mol Biol. 2006 Oct 06;7:33
pubmed: 17026756
Plants (Basel). 2021 Jun 22;10(7):
pubmed: 34206660
Cancer Res. 2004 Aug 1;64(15):5245-50
pubmed: 15289330
Sci Rep. 2021 Sep 21;11(1):18719
pubmed: 34548514
Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
pubmed: 12184808
Front Plant Sci. 2015 Mar 05;6:103
pubmed: 25798140
Plant Methods. 2020 Apr 25;16:58
pubmed: 32355504