Comparative transcriptomic analysis unveils candidate genes associated with sugarcane growth rate.
Saccharum
/ genetics
Plant Growth Regulators
/ metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant
Indoleacetic Acids
/ metabolism
Transcriptome
/ genetics
Plant Roots
/ growth & development
Cyclopentanes
/ metabolism
Gene Regulatory Networks
Oxylipins
/ metabolism
Genes, Plant
/ genetics
Bud
Gene networks
Hormone secretions
Sugarcane
Transcriptomics
Journal
Planta
ISSN: 1432-2048
Titre abrégé: Planta
Pays: Germany
ID NLM: 1250576
Informations de publication
Date de publication:
29 Oct 2024
29 Oct 2024
Historique:
received:
10
05
2024
accepted:
14
10
2024
medline:
30
10
2024
pubmed:
30
10
2024
entrez:
30
10
2024
Statut:
epublish
Résumé
Sugarcane (Saccharum spp.) growth is regulated by intricate gene networks and hormone secretions, positively correlating with sugarcane yield. There is a rising interest in exploring how the candidate genes found in sugarcane respond to plant growth. In this study, we simulated a typical growth environment to obtain accurate phenotypic data and screened for potential genes associated with plant growth through transcriptomics. Compared to Saccharum GuiTang 42, the other variety Saccharum GuiTang 44 exhibited earlier germination, a higher emergence rate, thicker pseudostems, taller plants, and a more extensive root system. The middle buds formed the greatest number of roots, followed by the lower and upper buds. Indole-3-acetic acid (IAA) and jasmonic acid effectively promoted bud development, while abscisic acid and trans-zeatin exhibited negative correlations with sugarcane bud growth. Transcriptome data from the upper, middle, and lower buds revealed 24,158 differentially expressed genes in all three comparisons, with MAPK signaling emerging as a critical pathway. The photosynthesis-antenna protein pathway is vital for middle and lower bud development during root germination. Lastly, key gene modules related to differences in hormone content between the two varieties were defined through weighted correlation network analysis and identified. The module significantly associated with IAA was enriched in pathways such as Proteasome and Protein processing in the endoplasmic reticulum, and the upregulation of key genes involved in this gene module had a highly significant positive correlation with bud outgrowth combined with IAA secretion. In conclusion, we have elucidated the pathways of hormones during sugarcane growth and the interactions between IAA and critical genes. These in-depth findings may guide modern sugarcane breeding.
Identifiants
pubmed: 39472317
doi: 10.1007/s00425-024-04555-3
pii: 10.1007/s00425-024-04555-3
doi:
Substances chimiques
Plant Growth Regulators
0
indoleacetic acid
6U1S09C61L
Indoleacetic Acids
0
Cyclopentanes
0
Oxylipins
0
jasmonic acid
6RI5N05OWW
Types de publication
Journal Article
Comparative Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
128Subventions
Organisme : National Natural Science Foundation
ID : 32360485
Organisme : National Natural Science Foundation
ID : 32360540
Organisme : National key research and development program
ID : 2022YFD2301100
Organisme : Natural Science Foundation of Guangxi Zhuang Autonomous Region
ID : 2021GXNSFAA220014
Organisme : Guangxi Natural Science Foundation Program
ID : 2023GXNSFAA026004
Organisme : National Modern Agricultural Industry Technology System Guangxi Sugarcane Innovation Team Cultivation Expert Pro-ject
ID : nycytxgxcxtd-2021-03-02
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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