Emulation of the structure of the Saposin protein fold by a lung surfactant peptide construct of surfactant Protein B.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2022
2022
Historique:
received:
04
07
2022
accepted:
14
10
2022
entrez:
3
11
2022
pubmed:
4
11
2022
medline:
8
11
2022
Statut:
epublish
Résumé
The three-dimensional structure of the synthetic lung Surfactant Protein B Peptide Super Mini-B was determined using an integrative experimental approach, including mass spectrometry and isotope enhanced Fourier-transform infrared (FTIR) spectroscopy. Mass spectral analysis of the peptide, oxidized by solvent assisted region-specific disulfide formation, confirmed that the correct folding and disulfide pairing could be facilitated using two different oxidative structure-promoting solvent systems. Residue specific analysis by isotope enhanced FTIR indicated that the N-terminal and C-terminal domains have well defined α-helical amino acid sequences. Using these experimentally derived measures of distance constraints and disulfide connectivity, the ensemble was further refined with molecular dynamics to provide a medium resolution, residue-specific structure for the peptide construct in a simulated synthetic lung surfactant lipid multilayer environment. The disulfide connectivity combined with the α-helical elements stabilize the peptide conformationally to form a helical hairpin structure that resembles critical elements of the Saposin protein fold of the predicted full-length Surfactant Protein B structure.
Identifiants
pubmed: 36327300
doi: 10.1371/journal.pone.0276787
pii: PONE-D-22-18838
pmc: PMC9632872
doi:
Substances chimiques
Saposins
0
IgA receptor
0
Pulmonary Surfactants
0
Peptides
0
Surface-Active Agents
0
Disulfides
0
Solvents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0276787Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK063491
Pays : United States
Déclaration de conflit d'intérêts
FW, AW, LG and the Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center hold a patent on Super Mini-B (US 8,563,683).
Références
PLoS One. 2013 Sep 02;8(9):e72821
pubmed: 24023779
Nat Protoc. 2010 Apr;5(4):725-38
pubmed: 20360767
J Chem Theory Comput. 2016 Jan 12;12(1):405-13
pubmed: 26631602
Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):589-94
pubmed: 18453694
N Engl J Med. 1993 Feb 11;328(6):406-10
pubmed: 8421459
Biochemistry. 2002 Aug 6;41(31):9852-62
pubmed: 12146951
Biopolymers. 1992 Dec;32(12):1695-702
pubmed: 1472652
J Chem Theory Comput. 2019 Jan 8;15(1):775-786
pubmed: 30525595
Biochemistry. 1991 Jul 16;30(28):6917-21
pubmed: 1648964
Am Rev Respir Dis. 1988 Oct;138(4):990-8
pubmed: 3059887
Biochemistry. 2007 Oct 2;46(39):11047-56
pubmed: 17845058
Nat Methods. 2019 Jul;16(7):587-594
pubmed: 31249407
Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10695-8
pubmed: 10485888
Protein Sci. 2003 Mar;12(3):520-31
pubmed: 12592022
Biophys J. 2003 Mar;84(3):1940-9
pubmed: 12609896
Mol Genet Metab. 2000 Sep-Oct;71(1-2):342-51
pubmed: 11001826
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
J Proteomics. 2014 May 30;103:216-26
pubmed: 24747305
Biochemistry. 2002 Dec 24;41(51):15296-303
pubmed: 12484768
Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):38-43
pubmed: 12518053
Protein Sci. 2008 Jan;17(1):95-106
pubmed: 18156469
Acc Chem Res. 2006 Mar;39(3):169-75
pubmed: 16548505
Rapid Commun Mass Spectrom. 2008;22(2):197-203
pubmed: 18088070
Nat Methods. 2015 Jan;12(1):7-8
pubmed: 25549265
Biochemistry. 1992 Sep 29;31(38):9169-76
pubmed: 1390703
Biophys J. 2002 Oct;83(4):2126-41
pubmed: 12324430
Biopolymers. 2000 Sep;54(3):180-5
pubmed: 10861379
Biophys J. 2003 Jul;85(1):340-9
pubmed: 12829488
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
Protein Sci. 2013 Jun;22(6):851-8
pubmed: 23526461
Cochrane Database Syst Rev. 2009 Apr 15;(2):CD007836
pubmed: 19370695
Protein Sci. 1998 Jun;7(6):1423-30
pubmed: 9655347
J Lipid Res. 1995 Aug;36(8):1653-63
pubmed: 7595087
J Pept Sci. 2007 Jan;13(1):37-43
pubmed: 17031869
Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11564-6
pubmed: 12195024
J Pept Res. 2005 Dec;66(6):364-74
pubmed: 16316452
Acta Paediatr. 2002;91(11):1174-8
pubmed: 12463314
Biochemistry. 1977 Jan 25;16(2):209-15
pubmed: 138432
Protein Sci. 1996 Aug;5(8):1662-75
pubmed: 8844855
Biochim Biophys Acta. 2016 Dec;1858(12):3113-3119
pubmed: 27664499
PLoS One. 2010 Jan 13;5(1):e8672
pubmed: 20084172
J Biomol Struct Dyn. 2012;30(6):773-83
pubmed: 22734588
J Pept Res. 2000 Apr;55(4):330-47
pubmed: 10798379
J Biomol NMR. 1996 Dec;8(4):477-86
pubmed: 9008363
Adv Protein Chem. 1986;38:181-364
pubmed: 3541539
Arch Biochem Biophys. 2001 Jan 15;385(2):338-47
pubmed: 11368015
Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7794-8
pubmed: 7644495
Expert Rev Respir Med. 2019 Jun;13(6):499-501
pubmed: 30817233
Biochem J. 1997 Sep 15;326 ( Pt 3):799-806
pubmed: 9307030
Protein Sci. 2004 Apr;13(4):1012-30
pubmed: 15044732
Nucleic Acids Res. 2015 Jul 1;43(W1):W174-81
pubmed: 25883148
Nat Struct Biol. 1997 Oct;4(10):793-5
pubmed: 9334742
J Mol Biol. 2003 Jan 10;325(2):355-65
pubmed: 12488100
Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):2908-12
pubmed: 22308394
Biochemistry. 2003 Dec 23;42(50):14729-40
pubmed: 14674747
Nucleic Acids Res. 2012 Jan;40(Database issue):D370-6
pubmed: 21890895
Neonatology. 2018;113(4):296-304
pubmed: 29428947
Pediatr Pulmonol. 2017 Jan;52(1):57-68
pubmed: 27362365
Biochemistry. 2006 Jun 20;45(24):7429-33
pubmed: 16768438
Int J Mol Sci. 2019 Aug 08;20(16):
pubmed: 31398818
Biochemistry. 1992 Feb 11;31(5):1280-95
pubmed: 1736987
Nature. 2021 Feb;590(7845):284-289
pubmed: 33461212
Biochim Biophys Acta. 2000 Jun 1;1466(1-2):179-86
pubmed: 10825441
Sci Rep. 2016 Feb 24;6:21571
pubmed: 26908371
J Biol Chem. 1986 Nov 25;261(33):15480-5
pubmed: 3096989
J Pediatr. 2020 Oct;225:90-96.e1
pubmed: 32553868
Eur Biophys J. 2013 Mar;42(2-3):209-22
pubmed: 22996193
Biochem J. 2005 Jul 15;389(Pt 2):249-57
pubmed: 15992358
Molecules. 2018 Feb 14;23(2):
pubmed: 29443946
J Exp Biol. 1999 Sep;202(Pt 18):2543-50
pubmed: 10460742