Amino-acyl tXNA as inhibitors or amino acid donors in peptide synthesis.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
11 11 2022
11 11 2022
Historique:
accepted:
21
10
2022
revised:
17
10
2022
received:
21
07
2022
pubmed:
10
11
2022
medline:
16
12
2022
entrez:
9
11
2022
Statut:
ppublish
Résumé
Xenobiotic nucleic acids (XNAs) offer tremendous potential for synthetic biology, biotechnology, and molecular medicine but their ability to mimic nucleic acids still needs to be explored. Here, to study the ability of XNA oligonucleotides to mimic tRNA, we synthesized three L-Ala-tXNAs analogs. These molecules were used in a non-ribosomal peptide synthesis involving a bacterial Fem transferase. We compared the ability of this enzyme to use amino-acyl tXNAs containing 1',5'-anhydrohexitol (HNA), 2'-fluoro ribose (2'F-RNA) and 2'-fluoro arabinose. L-Ala-tXNA containing HNA or 2'F-RNA were substrates of the Fem enzyme. The synthesis of peptidyl-XNA and the resolution of their structures in complex with the enzyme show the impact of the XNA on protein binding. For the first time we describe functional tXNA in an in vitro assay. These results invite to test tXNA also as substitute for tRNA in translation.
Identifiants
pubmed: 36350642
pii: 6814473
doi: 10.1093/nar/gkac1023
pmc: PMC9723616
doi:
Substances chimiques
Amino Acids
0
Nucleic Acids
0
Oligonucleotides
0
Peptides
0
RNA, Transfer, Ala
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11415-11425Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.
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