Proline-Rich Region II (PRR2) Plays an Important Role in Tau-Glycan Interaction: An NMR Study.


Journal

Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414

Informations de publication

Date de publication:
27 10 2022
Historique:
received: 23 02 2021
revised: 06 10 2022
accepted: 09 10 2022
entrez: 11 11 2022
pubmed: 12 11 2022
medline: 15 11 2022
Statut: epublish

Résumé

(1) Background: Prion-like transcellular spreading of tau pathology in Alzheimer's disease (AD) is mediated by tau binding to the cell-surface glycan heparan sulfate (HS). However, the structural determinants for tau-HS interaction are not well understood. (2) Methods and Results: Binding-site mapping using NMR showed two major binding regions in full-length tau responsible for heparin interaction. Thus, two tau constructs, tau PRR2* and tau R2*, were designed to investigate the molecular details at the tau-heparin binding interface. The 2D

Identifiants

pubmed: 36358923
pii: biom12111573
doi: 10.3390/biom12111573
pmc: PMC9687896
pii:
doi:

Substances chimiques

Fondaparinux J177FOW5JL
Heparitin Sulfate 9050-30-0
Heparin 9005-49-6
Proline 9DLQ4CIU6V
tau Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIH HHS
ID : 1RF1AG069039
Pays : United States
Organisme : NIA NIH HHS
ID : T32 AG057464
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL144970
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG069039
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG066493
Pays : United States

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Auteurs

Anqesha Murray (A)

Center for Biotechnology and Interdisciplinary Studies, Department of Chemistry and Chemical Biology, Departments of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, NY 12180, USA.

Lufeng Yan (L)

Center for Biotechnology and Interdisciplinary Studies, Department of Chemistry and Chemical Biology, Departments of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, NY 12180, USA.

James M Gibson (JM)

Center for Biotechnology and Interdisciplinary Studies, Department of Chemistry and Chemical Biology, Departments of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, NY 12180, USA.

Jian Liu (J)

Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27514, USA.

David Eliezer (D)

Program in Structural Biology, Department of Biochemistry, Weill Cornell Medical College, New York, NY 10065, USA.

Guy Lippens (G)

Toulouse Biotechnology Institute, CNRS, INRA, INSA, University of Toulouse, 31077 Toulouse, France.

Fuming Zhang (F)

Center for Biotechnology and Interdisciplinary Studies, Department of Chemistry and Chemical Biology, Departments of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, NY 12180, USA.

Robert J Linhardt (RJ)

Center for Biotechnology and Interdisciplinary Studies, Department of Chemistry and Chemical Biology, Departments of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, NY 12180, USA.

Jing Zhao (J)

Center for Biotechnology and Interdisciplinary Studies, Department of Chemistry and Chemical Biology, Departments of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, NY 12180, USA.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Chunyu Wang (C)

Center for Biotechnology and Interdisciplinary Studies, Department of Chemistry and Chemical Biology, Departments of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, NY 12180, USA.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

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Classifications MeSH