Immunoaffinity extraction followed by enzymatic digestion for the isolation and identification of proteins employing automated μSPE reactors and mass spectrometry.

Enzymes, Immobilized / chemistry Proteins / analysis Mass Spectrometry Silicon Dioxide / chemistry Solid Phase Extraction Digestion Trypsin / chemistry
IMER Immobilised enzyme Immunosorbent Mass spectrometry Protein analysis μSPE

Journal

Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327

Informations de publication

Date de publication:
Jul 2023
Historique:
received: 02 07 2022
accepted: 12 10 2022
revised: 01 09 2022
medline: 10 7 2023
pubmed: 13 11 2022
entrez: 12 11 2022
Statut: ppublish

Résumé

This work describes a novel automated and rapid method for bottom-up proteomics combining protein isolation with a micro-immobilised enzyme reactor (IMER). Crosslinking chemistry based on 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling was exploited to immobilise trypsin and antibodies onto customisable silica particles coated with carboxymethylated dextran (CMD). This novel silica-CMD solid-phase extraction material was characterised using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), conductometric titrations and enzymatic colorimetric assays. Micro-solid-phase extraction (μSPE) cartridges equipped with the modified CMD material were employed and integrated into an automated and repeatable workflow using a sample preparation workstation to achieve rapid and repeatable protein isolation and pre-concentration, followed by tryptic digestion producing peptide fragments that were identified by liquid chromatography mass spectrometry (LC-MS).

Identifiants

DOI: 10.1007/s00216-022-04381-0 PMID: 36369591 PMC: PMC10328895
pubmed: 36369591
doi: 10.1007/s00216-022-04381-0
pii: 10.1007/s00216-022-04381-0
pmc: PMC10328895
doi:

Substances chimiques

Enzymes, Immobilized 0
Proteins 0
Silicon Dioxide 7631-86-9
Trypsin EC 3.4.21.4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4173-4184

Informations de copyright

© 2022. The Author(s).

Références

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Auteurs

Karen Duong (K)

The Atomic Medicine Initiative, University of Technology Sydney, Ultimo, Australia.

Simin Maleknia (S)

School of Mathematical and Physical Sciences, Faculty of Science, University of Technology Sydney, Ultimo, Australia.

David Clases (D)

The Atomic Medicine Initiative, University of Technology Sydney, Ultimo, Australia.
Nano Micro LAB, Institute of Chemistry, University of Graz, Graz, Austria.

Andrew Minett (A)

ePrep Pty Ltd, Oakleigh, Victoria, 3166, Australia.

Matthew P Padula (MP)

School of Life Sciences and Proteomics Core Facility, Faculty of Science, University of Technology Sydney, Ultimo, Australia.

Philip A Doble (PA)

The Atomic Medicine Initiative, University of Technology Sydney, Ultimo, Australia.

Raquel Gonzalez de Vega (R)

The Atomic Medicine Initiative, University of Technology Sydney, Ultimo, Australia. raquel.gonzalez-de-vega@uni-graz.at.
TESLA-Analytical Chemistry, Institute of Chemistry, University of Graz, Graz, Austria. raquel.gonzalez-de-vega@uni-graz.at.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines immobilisées › Enzymes immobilisées › Immunoaffinity extraction followed by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Immunoaffinity extraction followed by...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Immunoaffinity extraction followed by...
questionsmedicales.fr › Produits chimiques inorganiques › Composés du silicium › Silice › Immunoaffinity extraction followed by...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Fractionnement chimique › Extraction en phase solide › Immunoaffinity extraction followed by...
questionsmedicales.fr › Phénomènes physiologiques de l'appareil buccodentaire et de l'appareil digestif › Phénomènes physiologiques de l'appareil digestif › Digestion › Immunoaffinity extraction followed by...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Protéases à sérine › Serine endopeptidases › Trypsine › Immunoaffinity extraction followed by...