Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.

Sugar Phosphates / metabolism Trehalose / metabolism Protein Serine-Threonine Kinases / genetics Plants / metabolism Signal Transduction Arabidopsis Proteins / genetics Gene Expression Regulation, Plant

Journal

Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677

Informations de publication

Date de publication:
11 2022
Historique:
received: 15 11 2021
accepted: 09 09 2022
entrez: 15 11 2022
pubmed: 16 11 2022
medline: 18 11 2022
Statut: ppublish

Résumé

The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited. Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking. Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation. At the intersection of this multi-dimensional interactome, we discovered a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins. Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1. Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.

Identifiants

DOI: 10.1038/s41477-022-01269-w PMID: 36376753
pubmed: 36376753
doi: 10.1038/s41477-022-01269-w
pii: 10.1038/s41477-022-01269-w
doi:

Substances chimiques

Sugar Phosphates 0
Trehalose B8WCK70T7I
Protein Serine-Threonine Kinases EC 2.7.11.1
Arabidopsis Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1245-1261

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer Nature Limited.

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Auteurs

Jelle Van Leene (J)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Dominique Eeckhout (D)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Astrid Gadeyne (A)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Caroline Matthijs (C)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Chao Han (C)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.
The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, China.

Nancy De Winne (N)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Geert Persiau (G)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Eveline Van De Slijke (E)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Freya Persyn (F)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Toon Mertens (T)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Wouter Smagghe (W)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Nathalie Crepin (N)

Laboratory for Molecular Plant Biology, Biology Department, KU Leuven, Heverlee-Leuven, Belgium.
KU Leuven Plant Institute-LPI, Heverlee-Leuven, Belgium.

Ellen Broucke (E)

Laboratory for Molecular Plant Biology, Biology Department, KU Leuven, Heverlee-Leuven, Belgium.
KU Leuven Plant Institute-LPI, Heverlee-Leuven, Belgium.

Daniël Van Damme (D)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Roman Pleskot (R)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.
Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech Republic.

Filip Rolland (F)

Laboratory for Molecular Plant Biology, Biology Department, KU Leuven, Heverlee-Leuven, Belgium.
KU Leuven Plant Institute-LPI, Heverlee-Leuven, Belgium.

Geert De Jaeger (G)

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium. geert.dejaeger@psb.vib-ugent.be.
VIB Center for Plant Systems Biology, Ghent, Belgium. geert.dejaeger@psb.vib-ugent.be.

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Classifications MeSH

questionsmedicales.fr › Glucides › Oses phosphates › Mapping of the plant SnRK1 kinase signalling...
questionsmedicales.fr › Glucides › Sucres › Diholoside › Tréhalose › Mapping of the plant SnRK1 kinase signalling...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Mapping of the plant SnRK1 kinase signalling...
questionsmedicales.fr › Eucaryotes › Plantes › Mapping of the plant SnRK1 kinase signalling...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Mapping of the plant SnRK1 kinase signalling...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › Mapping of the plant SnRK1 kinase signalling...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › Mapping of the plant SnRK1 kinase signalling...