Engineering a Conformationally Switchable Artificial Metalloprotein.
Journal
Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056
Informations de publication
Date de publication:
30 11 2022
30 11 2022
Historique:
pubmed:
16
11
2022
medline:
2
12
2022
entrez:
15
11
2022
Statut:
ppublish
Résumé
Many naturally occurring metalloenzymes are gated by rate-limiting conformational changes, and there exists a critical interplay between macroscopic structural rearrangements of the protein and subatomic changes affecting the electronic structure of embedded metallocofactors. Despite this connection, most artificial metalloproteins (ArMs) are prepared in structurally rigid protein hosts. To better model the natural mechanisms of metalloprotein reactivity, we have developed conformationally switchable ArMs (swArMs) that undergo a large-scale structural rearrangement upon allosteric effector binding. The swArMs reported here contain a Co(dmgH)
Identifiants
pubmed: 36378237
doi: 10.1021/jacs.2c08885
pmc: PMC10290874
mid: NIHMS1907245
doi:
Substances chimiques
Metalloproteins
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
21606-21616Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM138138
Pays : United States
Références
Acc Chem Res. 2016 Sep 20;49(9):1711-21
pubmed: 27529561
Biomol Concepts. 2011 Apr 1;2(1-2):53-64
pubmed: 25962019
Biochemistry. 1970 Jun 9;9(12):2387-93
pubmed: 5423257
Curr Opin Struct Biol. 2004 Aug;14(4):495-504
pubmed: 15313245
Science. 2018 Sep 14;361(6407):1098-1101
pubmed: 30213908
Adv Enzymol Relat Areas Mol Biol. 1975;43:219-410
pubmed: 892
Elife. 2013 Sep 10;2:e01456
pubmed: 24040512
J Mol Biol. 1989 Dec 20;210(4):849-57
pubmed: 2693743
J Am Chem Soc. 1972 Mar 22;94(6):1894-901
pubmed: 5016029
ACS Cent Sci. 2019 Jul 24;5(7):1120-1136
pubmed: 31404244
J Am Chem Soc. 2013 Oct 9;135(40):15077-84
pubmed: 24028405
J Am Chem Soc. 2009 Jul 15;131(27):9532-7
pubmed: 19583434
Annu Rev Biochem. 1999;68:779-819
pubmed: 10872466
J Mol Biol. 1998 Apr 24;278(1):219-29
pubmed: 9571045
Biochemistry. 1999 Jan 26;38(4):1234-42
pubmed: 9930983
J Bioenerg Biomembr. 1993 Apr;25(2):81-91
pubmed: 8389753
Chemistry. 2012 Jun 25;18(26):7984-99
pubmed: 22688954
Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6585-90
pubmed: 19346471
Nature. 2009 Aug 13;460(7257):855-62
pubmed: 19675646
FEBS Lett. 1999 Apr 23;449(2-3):191-5
pubmed: 10338130
Biochemistry. 2010 Mar 9;49(9):1893-902
pubmed: 20141110
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
pubmed: 20057044
Acc Chem Res. 2019 Mar 19;52(3):576-584
pubmed: 30830755
Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):262-7
pubmed: 26631748
Science. 2017 Jun 23;356(6344):1276-1280
pubmed: 28642436
Acta Crystallogr D Biol Crystallogr. 2009 Oct;65(Pt 10):1074-80
pubmed: 19770504
Methods Enzymol. 1997;276:307-26
pubmed: 27754618
Arch Biochem Biophys. 1971 Feb;142(2):715-7
pubmed: 4927558
J Am Chem Soc. 2010 May 26;132(20):6894-5
pubmed: 20429505
Science. 1985 Feb 22;227(4689):869-75
pubmed: 2857503
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):133-44
pubmed: 20124693
Biochem J. 2001 Apr 1;355(Pt 1):131-7
pubmed: 11256957
J Am Chem Soc. 2020 Sep 23;142(38):16334-16345
pubmed: 32871076
J Mol Biol. 1996 Sep 20;262(2):225-42
pubmed: 8831790
Methods Mol Biol. 2009;498:297-307
pubmed: 18988033
JACS Au. 2022 Jun 02;2(6):1252-1265
pubmed: 35783165
Appl Environ Microbiol. 2015 May 1;81(9):3205-17
pubmed: 25724962
Biochemistry. 1976 Nov 30;15(24):5399-406
pubmed: 187215
Biochemistry. 2013 Mar 26;52(12):2050-67
pubmed: 23488725
J Am Chem Soc. 2013 Jun 12;135(23):8585-93
pubmed: 23676140
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Biochemistry. 2003 Sep 2;42(34):10071-83
pubmed: 12939135
Biochemistry. 1972 Feb 29;11(5):842-7
pubmed: 4333944
Nat Commun. 2018 Aug 2;9(1):3038
pubmed: 30072686
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Sci Rep. 2017 Mar 16;7:44580
pubmed: 28300167
J Am Chem Soc. 2017 Dec 6;139(48):17289-17292
pubmed: 29117678
Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4328-33
pubmed: 25805820
Curr Protein Pept Sci. 2000 Dec;1(4):349-84
pubmed: 12369905
Acc Chem Res. 2011 Jan 18;44(1):47-57
pubmed: 20949947
Biochemistry. 1998 Mar 31;37(13):4459-64
pubmed: 9521765
Chem Rev. 2022 Jul 27;122(14):11974-12045
pubmed: 35816578
Trends Biochem Sci. 2019 Dec;44(12):1022-1040
pubmed: 31307903
J Am Chem Soc. 2017 Sep 13;139(36):12541-12549
pubmed: 28783336
Nature. 2007 Oct 25;449(7165):1078-82
pubmed: 17960247
J Biol Chem. 1998 Oct 16;273(42):27035-8
pubmed: 9765214
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Acc Chem Res. 2005 Jan;38(1):54-61
pubmed: 15654737
Biochemistry. 1994 Jun 21;33(24):7600-8
pubmed: 8011626