Rosemary essential oil and its components 1,8-cineole and α-pinene induce ROS-dependent lethality and ROS-independent virulence inhibition in Candida albicans.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2022
2022
Historique:
received:
15
06
2022
accepted:
18
10
2022
entrez:
16
11
2022
pubmed:
17
11
2022
medline:
19
11
2022
Statut:
epublish
Résumé
The essential oil from Rosmarinus officinalis L., a composite mixture of plant-derived secondary metabolites, exhibits antifungal activity against virulent candidal species. Here we report the impact of rosemary oil and two of its components, the monoterpene α-pinene and the monoterpenoid 1,8-cineole, against Candida albicans, which induce ROS-dependent cell death at high concentrations and inhibit hyphal morphogenesis and biofilm formation at lower concentrations. The minimum inhibitory concentrations (100% inhibition) for both rosemary oil and 1,8-cineole were 4500 μg/ml and 3125 μg/ml for α-pinene, with the two components exhibiting partial synergy (FICI = 0.55 ± 0.07). At MIC and 1/2 MIC, rosemary oil and its components induced a generalized cell wall stress response, causing damage to cellular and organelle membranes, along with elevated chitin production and increased cell surface adhesion and elasticity, leading to complete vacuolar segregation, mitochondrial depolarization, elevated reactive oxygen species, microtubule dysfunction, and cell cycle arrest mainly at the G1/S phase, consequently triggering cell death. Interestingly, the same oils at lower fractional MIC (1/8-1/4) inhibited virulence traits, including reduction of mycelium (up to 2-fold) and biofilm (up to 4-fold) formation, through a ROS-independent mechanism.
Identifiants
pubmed: 36383525
doi: 10.1371/journal.pone.0277097
pii: PONE-D-22-17200
pmc: PMC9668159
doi:
Substances chimiques
rosemary oil
8LGU7VM393
Eucalyptol
RV6J6604TK
alpha-pinene
JPF3YI7O34
Reactive Oxygen Species
0
Oils, Volatile
0
Antifungal Agents
0
Monoterpenes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0277097Informations de copyright
Copyright: © 2022 Shahina et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Eukaryot Cell. 2005 Oct;4(10):1697-711
pubmed: 16215177
Med Mycol. 2013 Nov;51(8):826-34
pubmed: 23718894
J Antimicrob Chemother. 2004 Jun;53(6):1081-5
pubmed: 15140856
J Antibiot (Tokyo). 2009 Dec;62(12):691-7
pubmed: 19876074
Eukaryot Cell. 2014 Feb;13(2):279-87
pubmed: 24363364
Antimicrob Agents Chemother. 2013 Aug;57(8):3498-506
pubmed: 23669379
Methods Mol Biol. 2015;1263:299-318
pubmed: 25618354
J Cell Sci. 2005 Jul 1;118(Pt 13):2935-47
pubmed: 15976451
Microbiol Rev. 1990 Sep;54(3):266-92
pubmed: 2215422
Rev Soc Bras Med Trop. 2016 Jul-Aug;49(4):465-72
pubmed: 27598633
PLoS Pathog. 2006 Jul;2(7):e63
pubmed: 16839200
Nanoscale. 2013 Feb 7;5(3):1105-15
pubmed: 23262781
Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):793-8
pubmed: 14718668
PLoS One. 2013 Oct 18;8(10):e76028
pubmed: 24204588
Cytoskeleton (Hoboken). 2014 Dec;71(12):707-18
pubmed: 25545749
J Cell Biol. 1997 Nov 17;139(4):985-94
pubmed: 9362516
Pharmaceuticals (Basel). 2013 Nov 25;6(12):1451-74
pubmed: 24287491
Annu Rev Phys Chem. 2019 Jun 14;70:199-218
pubmed: 30883272
Antimicrob Agents Chemother. 2012 Feb;56(2):960-71
pubmed: 22143530
Biophys J. 2003 Jun;84(6):3968-81
pubmed: 12770902
Mutat Res. 2006 Jul 14;606(1-2):27-38
pubmed: 16678471
J Microbiol. 2016 Mar;54(3):178-91
pubmed: 26920878
Nat Nanotechnol. 2017 Apr 6;12(4):295-307
pubmed: 28383040
Med Mycol. 2010 Nov;48(7):904-11
pubmed: 20392152
Food Microbiol. 2016 Feb;53(Pt B):24-30
pubmed: 26678126
Biofouling. 2013;29(1):87-96
pubmed: 23216018
J Antimicrob Chemother. 2013 Aug;68(8):1801-11
pubmed: 23612570
Rev Soc Bras Med Trop. 2014 May-Jun;47(3):389-91
pubmed: 25075493
Antimicrob Agents Chemother. 2014 Nov;58(11):6627-38
pubmed: 25155595
J Bacteriol. 1998 Mar;180(6):1418-24
pubmed: 9515908
Chem Rev. 2021 Mar 24;121(6):3390-3411
pubmed: 32441527
Mol Microbiol. 2009 Jan;71(2):505-19
pubmed: 19040629
Antimicrob Agents Chemother. 2018 Jan 25;62(2):
pubmed: 29203491
Mutagenesis. 2002 May;17(3):219-22
pubmed: 11971993
J Antimicrob Chemother. 2012 Mar;67(3):618-21
pubmed: 22167241
Mutat Res. 2005 Jan 6;569(1-2):13-27
pubmed: 15603750
Nucleic Acids Res. 2013 Jan;41(Database issue):D808-15
pubmed: 23203871
Front Cell Infect Microbiol. 2020 Apr 30;10:192
pubmed: 32426298
PLoS Pathog. 2014 Jun 19;10(6):e1004211
pubmed: 24945925
PLoS One. 2012;7(11):e50866
pubmed: 23226409
J Appl Microbiol. 2000 Jan;88(1):170-5
pubmed: 10735256
J Gen Microbiol. 1985 Oct;131(10):2581-9
pubmed: 2999296
Methods. 2008 Mar;44(3):222-8
pubmed: 18314052
J Clin Microbiol. 2004 Oct;42(10):4468-72
pubmed: 15472295
Biol Cell. 2003 Sep;95(6):335-42
pubmed: 14519550
Int J Antimicrob Agents. 2009 Oct;34(4):333-9
pubmed: 19376687
Phytomedicine. 2011 Oct 15;18(13):1181-90
pubmed: 21596542
J Clin Microbiol. 2004 Apr;42(4):1519-27
pubmed: 15070998
FEMS Yeast Res. 2014 Nov;14(7):1037-47
pubmed: 25130162
Fungal Biol Biotechnol. 2018 Feb 09;5:3
pubmed: 29456868
J Gen Microbiol. 1990 Jun;136(6):1067-75
pubmed: 2200842
Molecules. 2013 Aug 05;18(8):9334-51
pubmed: 23921795
Virulence. 2013 Feb 15;4(2):119-28
pubmed: 23302789
Cell. 1997 Sep 5;90(5):939-49
pubmed: 9298905
Elife. 2015 Aug 31;4:
pubmed: 26322385
Antimicrob Agents Chemother. 2003 Oct;47(10):3149-54
pubmed: 14506023
Nucleic Acids Res. 2017 Jan 4;45(D1):D592-D596
pubmed: 27738138
Acta Biochim Pol. 2014;61(1):115-21
pubmed: 24644554
Front Oncol. 2012 Jun 20;2:64
pubmed: 22737670
Cell Microbiol. 2006 Sep;8(9):1382-91
pubmed: 16848788
Med Res Rev. 2006 Mar;26(2):223-68
pubmed: 16331694
Molecules. 2017 Dec 05;22(12):
pubmed: 29206158
Biochim Biophys Acta. 2009 Apr;1793(4):650-63
pubmed: 18786576
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Microbiology (Reading). 1994 Feb;140 ( Pt 2):271-80
pubmed: 8180692
Eukaryot Cell. 2013 Feb;12(2):254-64
pubmed: 23243062
Chemosphere. 2015 Sep;135:453-61
pubmed: 25661029
Eukaryot Cell. 2011 Sep;10(9):1173-82
pubmed: 21764907
Sci Rep. 2017 Jul 18;7(1):5703
pubmed: 28720901
Acta Histochem Cytochem. 2007 Dec 18;40(5):131-7
pubmed: 18224244
Plants (Basel). 2020 Jun 26;9(6):
pubmed: 32604753
Crit Rev Microbiol. 2014 Feb;40(1):76-94
pubmed: 23445470
Mol Microbiol. 2013 Aug;89(4):649-59
pubmed: 23796134
J Clin Microbiol. 2003 Jul;41(7):2961-7
pubmed: 12843027
Methods Enzymol. 2014;542:243-62
pubmed: 24862270
Nat Rev Drug Discov. 2017 Sep;16(9):603-616
pubmed: 28496146
Nat Protoc. 2015 Feb;10(2):334-48
pubmed: 25633629
Pharmaceuticals (Basel). 2017 Nov 02;10(4):
pubmed: 29099084
Cell Calcium. 2016 Aug;60(2):102-7
pubmed: 26995056
FEMS Yeast Res. 2009 Oct;9(7):1013-28
pubmed: 19624749
FEMS Yeast Res. 2006 Jan;6(1):57-62
pubmed: 16423071
Mol Microbiol. 2003 Oct;50(1):167-81
pubmed: 14507372
Environ Toxicol Pharmacol. 2011 Jul;32(1):63-8
pubmed: 21787731
Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6910-5
pubmed: 27274080
J Agric Food Chem. 2000 Jun;48(6):2576-81
pubmed: 10888587
Appl Environ Microbiol. 1980 Aug;40(2):301-4
pubmed: 16345609
Mol Biol Cell. 2000 Dec;11(12):4241-57
pubmed: 11102521
Langmuir. 2005 Sep 27;21(20):9280-6
pubmed: 16171363
Clin Vaccine Immunol. 2009 Mar;16(3):430-2
pubmed: 19144791
Antimicrob Agents Chemother. 2015 Aug;59(8):4584-92
pubmed: 26014932
PLoS One. 2015 Feb 24;10(2):e0117695
pubmed: 25710475
Braz J Microbiol. 2013 Dec 10;44(3):813-21
pubmed: 24516422
Curr Opin Microbiol. 2006 Dec;9(6):588-94
pubmed: 17055772
Front Microbiol. 2018 Feb 01;9:44
pubmed: 29472899
Evid Based Complement Alternat Med. 2014;2014:125904
pubmed: 24719638
J Antimicrob Chemother. 2009 Dec;64(6):1219-25
pubmed: 19837714
Elife. 2020 Jun 05;9:
pubmed: 32501214
J Gen Microbiol. 1982 Sep;128(9):2195-8
pubmed: 6757384
Front Microbiol. 2015 Mar 09;6:176
pubmed: 25806026
Int J Antimicrob Agents. 2008 Jun;31(6):572-6
pubmed: 18440786
PLoS One. 2017 Feb 13;12(2):e0172228
pubmed: 28192489
Eukaryot Cell. 2008 Sep;7(9):1460-74
pubmed: 18586948
Food Chem Toxicol. 2008 Feb;46(2):446-75
pubmed: 17996351
Arch Biochem Biophys. 2002 Feb 15;398(2):213-20
pubmed: 11831852
Antimicrob Agents Chemother. 2010 Dec;54(12):5062-9
pubmed: 20921304
Int Rev Cell Mol Biol. 2013;302:41-185
pubmed: 23351710
Mycobiology. 2017 Mar;45(1):25-30
pubmed: 28435351
Mem Inst Oswaldo Cruz. 2016 Nov;111(11):697-702
pubmed: 27706381
Virulence. 2017 Feb 17;8(2):222-236
pubmed: 27820668
J Bacteriol. 1985 Mar;161(3):1195-1200
pubmed: 3156123
Phytomedicine. 2011 Dec 15;19(1):56-63
pubmed: 21924600
Proc Natl Acad Sci U S A. 2005 Jul 12;102(28):9878-83
pubmed: 15985553
PLoS One. 2018 Aug 29;13(8):e0203079
pubmed: 30157240