Detection of IMP-4 and SFO-1 co-producing ST51
Enterobacter hormaechei
IncN
ST51
blaIMP-4
blaSFO-1
genomics
multidrug-resistant
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2022
2022
Historique:
received:
20
07
2022
accepted:
13
10
2022
entrez:
17
11
2022
pubmed:
18
11
2022
medline:
22
11
2022
Statut:
epublish
Résumé
To explore the genetic characteristics of the IMP-4 and SFO-1 co-producing multidrug-resistant (MDR) clinical isolates, MALDI-TOF MS was used for species identification. Antibiotic resistance genes (ARGs) were tested by PCR and Sanger sequencing analysis. In addition to agar dilution, broth microdilution was used for antimicrobial susceptibility testing (AST). Whole-genome sequencing (WGS) analysis was conducted using the Illumina NovaSeq 6000 and Oxford Nanopore platforms. Annotation was performed by RAST on the genome. The phylogenetic tree was achieved using kSNP3.0. Plasmid characterization was conducted using S1-pulsed-field gel electrophoresis (S1-PFGE), Southern blotting, conjugation experiments, and whole genome sequencing (WGS). An in-depth study of the conjugation module was conducted using the OriTFinder website. The genetic context of YQ13422hy and YQ13530hy, two MDR strains of ST51 This observation revealed the dissemination of the
Identifiants
pubmed: 36389152
doi: 10.3389/fcimb.2022.998578
pmc: PMC9647121
doi:
Substances chimiques
Anti-Bacterial Agents
0
beta-Lactamases
EC 3.5.2.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
998578Informations de copyright
Copyright © 2022 Qiao, Ge, Xu, Guo, Liu, Li, Chen, Zheng and Gou.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Antimicrob Agents Chemother. 2000 May;44(5):1229-35
pubmed: 10770756
Front Cell Infect Microbiol. 2021 Nov 11;11:744431
pubmed: 34858870
Microb Drug Resist. 2022 Feb;28(2):180-184
pubmed: 34357815
Elife. 2021 Feb 26;10:
pubmed: 33634790
Int J Antimicrob Agents. 2020 Sep;56(3):106079
pubmed: 32634604
J Antimicrob Chemother. 2022 Jun 29;77(7):1873-1882
pubmed: 35412609
J Antimicrob Chemother. 2017 Jan;72(1):99-103
pubmed: 27609049
Antimicrob Agents Chemother. 1999 Feb;43(2):307-13
pubmed: 9925524
Virol Sin. 2015 Feb;30(1):11-8
pubmed: 25662887
Sci Rep. 2017 Dec 11;7(1):17321
pubmed: 29229976
Bioinformatics. 2015 Sep 1;31(17):2877-8
pubmed: 25913206
Environ Pollut. 2018 Apr;235:931-937
pubmed: 29358148
Future Microbiol. 2012 Jul;7(7):887-902
pubmed: 22827309
Antimicrob Agents Chemother. 2001 Mar;45(3):710-4
pubmed: 11181348
J Glob Antimicrob Resist. 2021 Dec;27:284-288
pubmed: 34775131
Front Microbiol. 2021 Dec 14;12:777054
pubmed: 34970236
Transbound Emerg Dis. 2021 Nov;68(6):3083-3088
pubmed: 33507616
Front Microbiol. 2017 Nov 28;8:2343
pubmed: 29326660
PLoS One. 2021 Jul 28;16(7):e0231119
pubmed: 34319973
Antimicrob Agents Chemother. 2015 Jul;59(7):4059-66
pubmed: 25918153
Front Microbiol. 2019 Jan 31;10:44
pubmed: 30766518
Microorganisms. 2021 Mar 10;9(3):
pubmed: 33801844
Antimicrob Agents Chemother. 2001 Aug;45(8):2368-71
pubmed: 11451699
Microb Genom. 2020 Jan;6(1):
pubmed: 31860437
Nat Commun. 2020 Jan 24;11(1):466
pubmed: 31980604
BMC Genomics. 2008 Feb 08;9:75
pubmed: 18261238
Antimicrob Agents Chemother. 1991 Jan;35(1):147-51
pubmed: 1901695
BMC Genomics. 2011 Aug 08;12:402
pubmed: 21824423
J Clin Microbiol. 2011 Mar;49(3):822-8
pubmed: 21227991
J Glob Antimicrob Resist. 2018 Jun;13:271-273
pubmed: 29432937
Infect Drug Resist. 2020 Feb 20;13:597-605
pubmed: 32110070
Front Microbiol. 2022 Jan 06;12:762509
pubmed: 35069468
Front Microbiol. 2019 Nov 22;10:2678
pubmed: 31824461
Environ Microbiol. 2021 Dec;23(12):7412-7431
pubmed: 33415808
J Glob Antimicrob Resist. 2022 Mar;28:254-263
pubmed: 35121164
Epidemiol Infect. 2014 Sep;142(9):1972-7
pubmed: 24252194
J Glob Antimicrob Resist. 2022 Sep;30:88-95
pubmed: 35489678
Microbiol Spectr. 2021 Oct 31;9(2):e0130521
pubmed: 34704778
Microbiol Spectr. 2015 Aug;3(4):
pubmed: 26350313
Antimicrob Agents Chemother. 2013 Aug;57(8):4072-6
pubmed: 23752513
J Antimicrob Chemother. 2017 Aug 1;72(8):2249-2258
pubmed: 28520983
Bioinformatics. 2011 Apr 1;27(7):1009-10
pubmed: 21278367