Differential phosphorylation of Clr4
chromosome segregation
fission yeast
histone methylation
meiosis
phosphorylation
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
09 01 2023
09 01 2023
Historique:
revised:
18
10
2022
received:
08
08
2022
accepted:
27
10
2022
pubmed:
22
11
2022
medline:
11
1
2023
entrez:
21
11
2022
Statut:
ppublish
Résumé
Methylation of histone H3 at lysine 9 (H3K9) is a hallmark of heterochromatin that plays crucial roles in gene silencing, genome stability, and chromosome segregation. In Schizosaccharomyces pombe, Clr4 mediates both di- and tri-methylation of H3K9. Although H3K9 methylation has been intensely studied in mitotic cells, its role during sexual differentiation remains unclear. Here, we map H3K9 methylation genome-wide during meiosis and show that constitutive heterochromatin temporarily loses H3K9me2 and becomes H3K9me3 when cells commit to meiosis. Cells lacking the ability to tri-methylate H3K9 exhibit meiotic chromosome segregation defects. Finally, the H3K9 methylation switch is accompanied by differential phosphorylation of Clr4 by the cyclin-dependent kinase Cdk1. Our results suggest that a conserved master regulator of the cell cycle controls the specificity of an H3K9 methyltransferase to prevent ectopic H3K9 methylation and to ensure faithful gametogenesis.
Identifiants
pubmed: 36408846
doi: 10.15252/embr.202255928
pmc: PMC9827552
doi:
Substances chimiques
Histones
0
Schizosaccharomyces pombe Proteins
0
Heterochromatin
0
Histone-Lysine N-Methyltransferase
EC 2.1.1.43
Cell Cycle Proteins
0
Methyltransferases
EC 2.1.1.-
clr4 protein, S pombe
EC 2.1.1.43
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e55928Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S018549
Pays : United Kingdom
Informations de copyright
© 2022 Friedrich Miescher Institute for Biomedical Research. Published under the terms of the CC BY 4.0 license.
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