Engineering Rieske oxygenase activity one piece at a time.
Journal
Current opinion in chemical biology
ISSN: 1879-0402
Titre abrégé: Curr Opin Chem Biol
Pays: England
ID NLM: 9811312
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
received:
07
09
2022
revised:
04
10
2022
accepted:
11
10
2022
pubmed:
22
11
2022
medline:
25
1
2023
entrez:
21
11
2022
Statut:
ppublish
Résumé
Enzyme engineering plays a central role in the development of biocatalysts for biotechnology, chemical and pharmaceutical manufacturing, and environmental remediation. Rational design of proteins has historically relied on targeting active site residues to confer a protein with desirable catalytic properties. However, additional "hotspots" are also known to exist beyond the active site. Structural elements such as subunit-subunit interactions, entrance tunnels, and flexible loops influence enzyme catalysis and serve as potential "hotspots" for engineering. For the Rieske oxygenases, which use a Rieske cluster and mononuclear iron center to catalyze a challenging set of reactions, these outside of the active site regions are increasingly being shown to drive catalytic outcomes. Therefore, here, we highlight recent work on structurally characterized Rieske oxygenases that implicates architectural pieces inside and outside of the active site as key dictators of catalysis, and we suggest that these features may warrant attention in efforts aimed at Rieske oxygenase engineering.
Identifiants
pubmed: 36410250
pii: S1367-5931(22)00112-0
doi: 10.1016/j.cbpa.2022.102227
pmc: PMC9939785
mid: NIHMS1873514
pii:
doi:
Substances chimiques
Oxygenases
EC 1.13.-
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
102227Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM138271
Pays : United States
Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Jennifer Bridwell-Rabb is a co-editor for the Biocatalysis and Biotransformation issue.
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