Tip60-mediated H2A.Z acetylation promotes neuronal fate specification and bivalent gene activation.
Ascl1
H2A.Z acetylation
H3K4me3
Tip60/Kat5
bivalent chromatin
cell fate
gene activation
neurogenesis
reprogramming
transcription
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
15 12 2022
15 12 2022
Historique:
received:
13
04
2022
revised:
28
08
2022
accepted:
31
10
2022
pmc-release:
15
12
2023
pubmed:
24
11
2022
medline:
21
12
2022
entrez:
23
11
2022
Statut:
ppublish
Résumé
Cell lineage specification is accomplished by a concerted action of chromatin remodeling and tissue-specific transcription factors. However, the mechanisms that induce and maintain appropriate lineage-specific gene expression remain elusive. Here, we used an unbiased proteomics approach to characterize chromatin regulators that mediate the induction of neuronal cell fate. We found that Tip60 acetyltransferase is essential to establish neuronal cell identity partly via acetylation of the histone variant H2A.Z. Despite its tight correlation with gene expression and active chromatin, loss of H2A.Z acetylation had little effect on chromatin accessibility or transcription. Instead, loss of Tip60 and acetyl-H2A.Z interfered with H3K4me3 deposition and activation of a unique subset of silent, lineage-restricted genes characterized by a bivalent chromatin configuration at their promoters. Altogether, our results illuminate the mechanisms underlying bivalent chromatin activation and reveal that H2A.Z acetylation regulates neuronal fate specification by establishing epigenetic competence for bivalent gene activation and cell lineage transition.
Identifiants
pubmed: 36417913
pii: S1097-2765(22)01064-4
doi: 10.1016/j.molcel.2022.11.002
pmc: PMC9779922
mid: NIHMS1853175
pii:
doi:
Substances chimiques
Histones
0
Chromatin
0
Nucleosomes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
4627-4646.e14Subventions
Organisme : NIMH NIH HHS
ID : R01 MH115999
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM139569
Pays : United States
Organisme : NIH HHS
ID : S10 OD018220
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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