Production of C20 9S- and C22 11S-hydroxy fatty acids by cells expressing Shewanella hanedai arachidonate 9S-lipoxygenase.
Arachidonate 9S-lipoxygenase
C20- and C22-polyunsaturated fatty acids
Characterization
Hydroxy fatty acids
Shewanella hanedai
Whole-cell conversion
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
received:
08
06
2022
accepted:
08
11
2022
revised:
24
10
2022
pubmed:
29
11
2022
medline:
17
12
2022
entrez:
28
11
2022
Statut:
ppublish
Résumé
The putative lipoxygenase (LOX) from the proteobacterium Shewanella hanedai was determined to be an 82 kDa monomeric enzyme by SDS-PAGE and gel filtration chromatography analysis. LOX was identified as a single-dioxygenating arachidonate (ARA) 9S-LOX by analyzing ARA-derived bioconversion products using high-performance liquid chromatography with reverse-, normal-, and chiral-phase columns and evaluating kinetic parameters for C20- and C22-polyunsaturated fatty acids (PUFAs). The catalytic efficiency (k
Identifiants
pubmed: 36441205
doi: 10.1007/s00253-022-12285-3
pii: 10.1007/s00253-022-12285-3
doi:
Substances chimiques
Arachidonate Lipoxygenases
EC 1.13.11.-
Fatty Acids
0
Fatty Acids, Unsaturated
0
Lipoxygenase
EC 1.13.11.12
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
247-260Subventions
Organisme : Ministry of Science and ICT, Republic of Korea
ID : 2020R1A4A1018648
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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