Formation of subcellular compartments by condensation-prone protein OsJAZ2 in Oryza sativa and Nicotiana benthamiana leaf cells.
Condensation-prone protein
Intrinsically disordered protein
JAZ
Jasmonate
Membraneless organelles
Phase separation
Journal
Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
received:
17
07
2022
accepted:
21
11
2022
pubmed:
1
12
2022
medline:
3
3
2023
entrez:
30
11
2022
Statut:
ppublish
Résumé
OsJAZ2 protein has a propensity to form condensates, possibly by multivalent interactions, and can be used to construct artificial compartments in plant cells. Eukaryotic cells contain various membraneless organelles, which are compartments consisting of proteinaceous condensates formed by phase separation. Such compartments are attractive for bioengineering and synthetic biology, because they can modify cellular function by the enrichment of molecules of interest and providing an orthogonal reaction system. This study reports that Oryza sativa JAZ2 protein (OsJAZ2) is an atypical jasmonate signalling regulator that can form large condensates in both the nucleus and cytosol of O. sativa cells. TIFY and Jas domains and low-complexity regions contribute to JAZ2 condensation, possibly by multivalent interaction. Fluorescence recovery after photobleaching (FRAP) analysis suggests that JAZ2 condensates form mostly gel-like or solid compartments, but can also be in a liquid-like state. Deletion of the N-terminal region or the TIFY domain of JAZ2 causes an increase in the mobile fraction of JAZ2 condensates, moderately. Moreover, JAZ2 can also form liquid-like condensates when expressed in Nicotiana benthamiana cells. The recombinant JAZ2 fused to the green fluorescent protein (GFP) forms condensate in vitro, suggesting that the intermolecular interaction of JAZ2 molecules is a driving force for condensation. These results suggest the potential use of JAZ2 condensates to construct artificial membraneless organelles in plant cells.
Identifiants
pubmed: 36449075
doi: 10.1007/s00299-022-02955-x
pii: 10.1007/s00299-022-02955-x
doi:
Substances chimiques
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
269-286Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP16K08140
Organisme : Japan Society for the Promotion of Science
ID : JP22K05427
Organisme : National Institute of Advanced Industrial Science and Technology
ID : NU-AIST alliance project
Organisme : Japan Science and Technology Agency
ID : JPMJSP2125
Organisme : Ministry of Education, Culture, Sports, Science and Technology
ID : Graduate Program of Transformative Chem-Bio Research
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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