Simultaneous quantification of tryptophan metabolites by liquid chromatography tandem mass spectrometry during early human pregnancy.
liquid chromatography
mass spectrometry
pregnancy
tryptophan metabolism
Journal
Clinical chemistry and laboratory medicine
ISSN: 1437-4331
Titre abrégé: Clin Chem Lab Med
Pays: Germany
ID NLM: 9806306
Informations de publication
Date de publication:
23 02 2023
23 02 2023
Historique:
received:
10
08
2022
accepted:
22
11
2022
pubmed:
3
12
2022
medline:
28
1
2023
entrez:
2
12
2022
Statut:
epublish
Résumé
In this study we describe the development and validation of a liquid chromatography mass spectrometry method (LC-MS/MS) to quantify five tryptophan (TRP) metabolites within the kynurenine- and serotonin pathway and apply the method to serum samples of women in the first trimester of pregnancy. A secondary aim was to investigate the correlation between body mass index (BMI) and the five analytes. A LC-MS/MS was developed for the analysis of TRP, kynurenine (KYN), 5-hydroxytryptophan (5-HTP), hydroxytryptamine (5-HT), and 5-hydroxyindole acetic acid (5-HIAA). Serum samples (n=374) were analyzed of pregnant women (median gestational age: 8 ± 2 weeks) participating in a subcohort of the Rotterdam Periconceptional Cohort (Predict study). The LC-MS/MS method provided satisfactory separation of the five analytes (7 min run). For all analytes R The LC-MS/MS method is able to accurately quantify kynurenine- and serotonin pathway metabolites in pregnant women, providing an opportunity to investigate the role of the TRP metabolism in the (patho)physiology of pregnancy.
Identifiants
pubmed: 36458576
pii: cclm-2022-0790
doi: 10.1515/cclm-2022-0790
doi:
Substances chimiques
Kynurenine
343-65-7
Tryptophan
8DUH1N11BX
Serotonin
333DO1RDJY
5-Hydroxytryptophan
C1LJO185Q9
Hydroxyindoleacetic Acid
54-16-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
442-451Informations de copyright
© 2022 the author(s), published by De Gruyter, Berlin/Boston.
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