Neutrophilic granulocyte-derived B-cell activating factor supports B cells in skin lesions in hidradenitis suppurativa.
Acne inversa
B cell
Cibersort
G-CSF
IL-17
RNA sequencing
TNF-α
TNFSF13B
granulocyte
plasma cell
tertiary lymphoid structures
Journal
The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
received:
25
03
2022
revised:
17
09
2022
accepted:
20
10
2022
medline:
11
4
2023
pubmed:
9
12
2022
entrez:
8
12
2022
Statut:
ppublish
Résumé
Hidradenitis suppurativa (HS) is a chronic inflammatory disease characterized by painful inflamed nodules, abscesses, and pus-draining tunnels appearing in axillary, inguinal, and perianal skin areas. HS lesions contain various types of immigrated immune cells. This study aimed to characterize mediators that support lesional B/plasma cell persistence in HS. Skin samples from several cohorts of HS patients and control cohorts were assessed by mRNA sequencing, quantitative PCR on reverse-transcribed RNA, flow cytometry, and immunohistofluorescence. Blood plasma and cultured skin biopsy samples, keratinocytes, dermal fibroblasts, neutrophilic granulocytes (neutrophils), monocytes, and B cells were analyzed. Complex systems biology approaches were used to evaluate bulk and single-cell RNA sequencing data. Proportions of B/plasma cells, neutrophils, CD8 Neutrophil-derived BAFF supports B/plasma cell persistence and function in HS lesions.
Sections du résumé
BACKGROUND
Hidradenitis suppurativa (HS) is a chronic inflammatory disease characterized by painful inflamed nodules, abscesses, and pus-draining tunnels appearing in axillary, inguinal, and perianal skin areas. HS lesions contain various types of immigrated immune cells.
OBJECTIVE
This study aimed to characterize mediators that support lesional B/plasma cell persistence in HS.
METHODS
Skin samples from several cohorts of HS patients and control cohorts were assessed by mRNA sequencing, quantitative PCR on reverse-transcribed RNA, flow cytometry, and immunohistofluorescence. Blood plasma and cultured skin biopsy samples, keratinocytes, dermal fibroblasts, neutrophilic granulocytes (neutrophils), monocytes, and B cells were analyzed. Complex systems biology approaches were used to evaluate bulk and single-cell RNA sequencing data.
RESULTS
Proportions of B/plasma cells, neutrophils, CD8
CONCLUSION
Neutrophil-derived BAFF supports B/plasma cell persistence and function in HS lesions.
Identifiants
pubmed: 36481267
pii: S0091-6749(22)01621-9
doi: 10.1016/j.jaci.2022.10.034
pii:
doi:
Substances chimiques
B-Cell Activating Factor
0
Interleukin-4
207137-56-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1015-1026Informations de copyright
Copyright © 2022. Published by Elsevier Inc.