Discovery and structure of a widespread bacterial ABC transporter specific for ergothioneine.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
08 12 2022
08 12 2022
Historique:
received:
05
05
2022
accepted:
22
11
2022
entrez:
9
12
2022
pubmed:
10
12
2022
medline:
15
12
2022
Statut:
epublish
Résumé
L-Ergothioneine (ET), the 2-thioimidazole derivative of trimethylhistidine, is biosynthesized by select fungi and bacteria, notably Mycobacterium tuberculosis, and functions as a scavenger of reactive oxygen species. The extent to which ET broadly functions in bacterial cells unable to synthesize it is unknown. Here we show that spd_1642-1643 in Streptococcus pneumoniae, a Gram-positive respiratory pathogen, encodes an ET uptake ATP-binding cassette (ABC) transporter, designated EgtU. The solute binding domain (SBD) of EgtU, EgtUC, binds ET with high affinity and exquisite specificity in a cleft between the two subdomains, with cation-π interactions engaging the betaine moiety and a network of water molecules that surround the thioimidazole ring. EgtU is highly conserved among known quaternary amine compound-specific transporters and widely distributed in Firmicutes, including the human pathogens Listeria monocytogenes, as BilEB, Enterococcus faecalis and Staphylococcus aureus. ET increases the chemical diversity of the low molecular weight thiol pool in Gram-positive human pathogens and may contribute to antioxidant defenses in the infected host.
Identifiants
pubmed: 36481738
doi: 10.1038/s41467-022-35277-3
pii: 10.1038/s41467-022-35277-3
pmc: PMC9732360
doi:
Substances chimiques
ATP-Binding Cassette Transporters
0
Ergothioneine
BDZ3DQM98W
Bacterial Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
7586Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM118157
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM131994
Pays : United States
Informations de copyright
© 2022. The Author(s).
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