Unbiased Quantification of Golgi Scattering and Golgi-Centrosome Association.
Centrosome separation
Golgi fragmentation
Golgi volume
Interphase
MATLAB script
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
pmc-release:
01
01
2024
entrez:
13
12
2022
pubmed:
14
12
2022
medline:
16
12
2022
Statut:
ppublish
Résumé
The vertebrate Golgi complex is a large dynamic organelle which undergoes morphological changes and fragmentation both as a part of normal physiological dynamics and under disease conditions. The Golgi is known to have a functionally important relationship with the centrosome. The extent of the spatial association between these two organelles varies in a dynamic and regulated manner. It is essential to have a reliable unbiased approach to evaluate Golgi volume, Golgi extension/scattering in the 3D cell space, and spatial association of the Golgi with the centrosome. It is also important that each of these features is evaluated by a simple metric, one measurement per cell, so that the variability and deviations in the cell population can be easily assessed. Here, we present an approach to analyze confocal microscopy image stacks to easily measure Golgi volume, scattering, and association with the centrosome. The approach is based on a custom MATLAB script, provided here as a supplement, and also uses widely available software (ImageJ and/or Imaris). The output of the script is a table with the following parameters: Golgi volume in voxels, Golgi volume in μm
Identifiants
pubmed: 36512235
doi: 10.1007/978-1-0716-2639-9_31
pmc: PMC9844073
mid: NIHMS1859457
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
529-541Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK106228
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM062459
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM127098
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130298
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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