A bacterial Argonaute from Tepiditoga spiralis with the ability of RNA guided plasmid cleavage.
Endonuclease
Plasmid cleavage
Prokaryotic argonaute proteins
RNA guides
Tepiditoga spiralis
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
15 01 2023
15 01 2023
Historique:
received:
10
11
2022
revised:
29
11
2022
accepted:
29
11
2022
pubmed:
14
12
2022
medline:
11
1
2023
entrez:
13
12
2022
Statut:
ppublish
Résumé
The eukaryotic Argonaute proteins (eAgos) play an important role in the RNA interference pathway. The function and mechanism of prokaryotic Argonaute proteins (pAgos) in vivo are still unclear although the structure of pAgos and eAgos are highly homologous. Most of the reported pAgos have a preference for 5'P-gDNA, but MpAgo originated from bacteria Marinitoga piezophila preferentially uses 5'OH-gRNA to target DNA and RNA. To enrich our knowledge of this type of Argonaute proteins, here we report an Argonaute protein derived from Tepiditoga spiralis (TsAgo). Like MpAgo, TsAgo has a preference for 5'OH-gRNA. Meanwhile, TsAgo has DNA and RNA cleavage activity in presence of Mn
Identifiants
pubmed: 36512847
pii: S0006-291X(22)01655-2
doi: 10.1016/j.bbrc.2022.11.097
pii:
doi:
Substances chimiques
RNA
63231-63-0
Argonaute Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
157-163Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Lixin Ma reports a relationship with Hubei University that includes: employment. Lixin Ma has patent #202211260452.4 pending to China National Intellectual Property Administration.