Chromosome evolution of Escherichia coli Nissle 1917 for high-level production of heparosan.
E. coli Nissle 1917
bioengineered heparin
chromosome evolution
fed-batch fermentation
heparosan
kps locus
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
revised:
11
12
2022
received:
19
09
2022
accepted:
17
12
2022
pubmed:
22
12
2022
medline:
17
3
2023
entrez:
21
12
2022
Statut:
ppublish
Résumé
Heparosan is a crucial-polysaccharide precursor for the chemoenzymatic synthesis of heparin, a widely used anticoagulant drug. Presently, heparosan is mainly extracted with the potential risk of contamination from Escherichia coli strain K5, a pathogenic bacterium causing urinary tract infection. Here, a nonpathogenic probiotic, E. coli strain Nissle 1917 (EcN), was metabolically engineered to carry multiple copies of the 19-kb kps locus and produce heparosan to 9.1 g/L in fed-batch fermentation. Chromosome evolution driven by antibiotics was employed to amplify the kps locus, which governed the synthesis and export of heparosan from EcN at 21 mg L
Substances chimiques
heparosan
0
Heparin
9005-49-6
Disaccharides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1081-1096Informations de copyright
© 2022 Wiley Periodicals LLC.
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