Molecular cloning and functional characterization of melanocortin-3 receptor in grass carp (Ctenopharyngodon idella).

Animals Humans Carps / genetics Cloning, Molecular Fish Proteins / genetics HEK293 Cells Phylogeny Receptor, Melanocortin, Type 3 / genetics
Grass carp MAPK/ERK Melanocortin-3-receptor (MC3R) cAMP

Journal

Fish physiology and biochemistry
ISSN: 1573-5168
Titre abrégé: Fish Physiol Biochem
Pays: Netherlands
ID NLM: 100955049

Informations de publication

Date de publication:
Feb 2023
Historique:
accepted: 13 12 2022
received: 11 06 2022
entrez: 22 12 2022
medline: 22 2 2023
pubmed: 23 12 2022
Statut: ppublish

Résumé

The melanocortin-3-receptor (MC3R) plays an important role in mammals' food intake and energy homeostasis. However, its physiological role in bony fishes, such as grass carp, has not been well understood. This study reports the molecular cloning, tissue distribution, and pharmacological characterization of grass carp melanocortin-3-receptor (ciMC3R). Phylogenetic and chromosomal synteny analyses indicated that ciMC3R was closest to cyprinid fishes in evolution. Quantitative PCR experiments revealed that the mRNA of ciMC3R was highly expressed in the brain of grass carp. The cytological function of ciMC3R was investigated by the co-transfection of pcDNA3.1-ciMC3R and the signal-pathway-specific luciferase into the HEK293T cells. Results revealed that the four agonists, α-MSH, β-MSH, ACTH, and NDP-MSH, potentiate the activation of ciMC3R and further increase the production of cAMP and upregulate the MAPK/ERK signaling, respectively. Our study will provide basic data for exploring the physiological functions of grass carp MC3R, especially in energy homeostasis and food intake.

Identifiants

DOI: 10.1007/s10695-022-01164-3 PMID: 36547499
pubmed: 36547499
doi: 10.1007/s10695-022-01164-3
pii: 10.1007/s10695-022-01164-3
doi:

Substances chimiques

Fish Proteins 0
Receptor, Melanocortin, Type 3 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

155-167

Subventions

Organisme : National Key R&D Program of China
ID : 2019YFD0900200
Organisme : National Natural Science Foundation of China
ID : 36431502180
Organisme : Shaanxi Provincial Water Conservancy Science and Technology Fund
ID : 2016slkj-16

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer Nature B.V.

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Auteurs

Haolin Mo (H)

College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China.

Huixia Yu (H)

College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China.

Yang Li (Y)

College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China. yangli2001@nwsuaf.edu.cn.

Timothy P C Ezeorba (TPC)

Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Ihe Nsukka, Nsukka, 41001, Nigeria.

Zhihao Zhang (Z)

College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China.

Mingxin Yao (M)

College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China.

Jiajia Yu (J)

College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China.

Dongmei Xiong (D)

College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China.

Haixia Liu (H)

College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China.

Lixin Wang (L)

College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China. fisherwanglx@nwsuaf.edu.cn.

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questionsmedicales.fr › Eucaryotes › Animaux › Molecular cloning and functional...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Molecular cloning and functional...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Cypriniformes › Cyprinidae › Carpes (poisson) › Molecular cloning and functional...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Clonage moléculaire › Molecular cloning and functional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de poisson › Molecular cloning and functional...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Molecular cloning and functional...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Molecular cloning and functional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs peptidiques › Récepteur hormone hypophysaire › Récepteur corticotrophine › Récepteurs à la mélanocortine › Récepteur de la mélanocortine de type 3 › Molecular cloning and functional...