Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis.
Ustilago maydis
Zea mays
aspartic protease
protease
secreted protein
spore germination
sporulation
Journal
Yeast (Chichester, England)
ISSN: 1097-0061
Titre abrégé: Yeast
Pays: England
ID NLM: 8607637
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
revised:
19
12
2022
received:
20
08
2022
accepted:
21
12
2022
pubmed:
24
12
2022
medline:
7
2
2023
entrez:
23
12
2022
Statut:
ppublish
Résumé
Ustilago maydis expresses a number of proteases during its pathogenic lifecycle. Some of the proteases including both intracellular and extracellular ones have previously been shown to influence the virulence of the pathogen. However, any role of secreted proteases in the sporulation process of U. maydis have not been explored earlier. In this study we have investigated the biological function of one such secreted protease, Ger1 belonging to aspartic protease A1 family. An assessment of the real time expression of ger1 revealed an infection specific expression of the protein especially during late phases of infection. We also evaluated any contribution of the protein in the pathogenicity of the fungus. Our data revealed an involvement of Ger1 in the sporulation and spore germination processes of U. maydis. Ger1 also showed positive influence on the pathogenicity of the fungus and accordingly the ger1 deletion mutant exhibited reduced pathogenicity. The study also demonstrated the protease activity associated with Ger1 to be essential for its biological function. Fluorescence microscopy of maize plants infected with U. maydis cells expressing Ger1-mcherry-HA also revealed that Ger1 is efficiently secreted within maize apoplast.
Substances chimiques
Aspartic Acid Proteases
EC 3.4.-
Fungal Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102-116Informations de copyright
© 2022 John Wiley & Sons Ltd.
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