New Hydrogels Based on Agarose/Phytagel and Peptides.
Agarose
Phytagel
hydrogels
macromolecule
multicomponent gel
peptides
polysaccharide
Journal
Macromolecular bioscience
ISSN: 1616-5195
Titre abrégé: Macromol Biosci
Pays: Germany
ID NLM: 101135941
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
revised:
19
12
2022
received:
25
10
2022
pubmed:
25
12
2022
medline:
17
3
2023
entrez:
24
12
2022
Statut:
ppublish
Résumé
Short aromatic peptide derivatives, i.e., peptides or amino acids modified with aromatic groups, such as 9-fluorenylmethoxycarbonyl (Fmoc), can self-assemble into extracellular matrix-like hydrogels due to their nanofibrillar architecture. Among different types of amino acids, lysine (Lys) and glycine (Gly) are involved in multiple physiological processes, being key factors in the proper growth of cells, carnitine production, and collagen formation. The authors have previously successfully presented the possibility of obtaining supramolecular gels based on Fmoc-Lys-Fmoc and short peptides such as Fmoc-Gly-Gly-Gly in order to use them as a substrate for cell cultures. This paper investigates how the introduction of a gelling polymer can influence the properties of the network as well as the compatibility of the resulting materials with different cell types. A series of hydrogel compositions consisting of combinations of Fmoc-Lys-Fmoc and Fmoc-Gly-Gly-Gly with Agarose and Phytagel are thus obtained. All compositions form structured gels as shown by rheological studies and scanning electron microscopy. Fourier transform infrared spectroscopy analysis evidences the formation of H-bonds between the polysaccharides and amino acids or short peptides. Moreover, all gels exhibit good cell viability on fibroblasts as demonstrated by a live-dead staining test and good in vivo biocompatibility, which highlights the great potential of these biomaterials for biomedical applications.
Identifiants
pubmed: 36565479
doi: 10.1002/mabi.202200451
doi:
Substances chimiques
Hydrogels
0
Sepharose
9012-36-6
Peptides
0
Amino Acids
0
Biocompatible Materials
0
Lysine
K3Z4F929H6
Glycine
TE7660XO1C
Fluorenes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2200451Informations de copyright
© 2022 Wiley-VCH GmbH.
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