Design and Development of Extracellular Matrix Protein-Based Microcapsules as Tools for Bacteria Investigation.
ECM-protein microcapsules
droplet-based microfluidics
filamentous bacteria
synthetic infection models
Journal
Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
revised:
19
12
2022
received:
29
10
2022
medline:
27
4
2023
pubmed:
5
1
2023
entrez:
4
1
2023
Statut:
ppublish
Résumé
The extracellular matrix (ECM) plays an immense role in the homeostasis of tissues and organs, can function as a barrier for infectious agents, but is also exploited by pathogens during infection. Therefore, the development of well-defined 3D ECM models in the form of microcapsules to elucidate the interactions between ECM components and pathogens in confinement and study disease infectivity is important, albeit challenging. Current limitations are mainly attributed to the lack of biocompatible methods for the production of protein-based microcapsules. Herein, hollow ECM-based microcapsules from laminin-111 or laminin-111/collagen IV are generated to investigate the behavior of organisms within confined 3D extracellular matrices. Microcapsules are created using water-in-oil emulsion droplets stabilized by block copolymer surfactants as templates for the charge-mediated attraction of laminin or laminin-collagen proteins to the droplets' inner periphery, allowing for the formation of modular ECM-based microcapsules with tunable biophysical and biochemical properties and organism encapsulation. The release of E. coli-laden ECM-based protein microcapsules into a physiological environment revealed differences in the dynamic behavior of E. coli depending on the constitution of the surrounding ECM protein matrix. The developed ECM-based protein microcapsules have the potential to be implemented in several biomedical applications, including the design of in vitro infection models.
Identifiants
pubmed: 36599129
doi: 10.1002/adhm.202202789
doi:
Substances chimiques
Laminin
0
Extracellular Matrix Proteins
0
Capsules
0
Collagen Type IV
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2202789Subventions
Organisme : European Research Council
ID : 294852
Pays : International
Informations de copyright
© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.
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